Rabbit Troponin C Polyclonal Antibody | anti-TNNC1 antibody

Anti-Troponin C Antibody, Rabbit Polyclonal

Cat. Num. AAA8103462

• Gene Names: Tnnc1; TnC; cTnC; cTnI; tncc; AI874626
• Reactivity: Mouse, Human; Predicted to React with: Bovin, Chick, Pig, Rabbit (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Troponin C; Polyclonal Antibody; Anti-Troponin C Antibody; Rabbit Polyclonal; Troponin C/TNNC1 Antibody; Rabbit PAb; Antigen Affinity Purified; troponin C type 1 (slow); Anti-AI874626 Antibody; Anti-cTnC Antibody; Anti-cTnI Antibody; Anti-TnC Antibody; Anti-tncc Antibody; anti-TNNC1 antibody

• Host: Rabbit
• Reactivity: Mouse, Human; Predicted to React with: Bovin, Chick, Pig, Rabbit (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Troponin C
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TNNC1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse Troponin C/TNNC1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse Troponin C/TNNC1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining TNNC1 in mouse skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining TNNC1 in mouse heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining TNNC1 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-TNNC1 antibody

Troponin I, also known as TNNC1, is part of the troponin complex. This complex contains 3 subunits: troponin I (TnI), troponin T (TnT) and troponin C (TnC). Troponin I is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle.

References

Kalaji FR. et al., 2012, Saudi J Kidney Dis Transpl. 23 (5): 939-45.Wijnker PJ. et al., 2013, Am J Physiol Heart Circ Physiol. 304 (2): H260-8.Solaro RJ. et al., 2013, Circ Res. 112 (2): 355-66.

NCBI and Uniprot Product Information

• NCBI GI #: 6678369
• NCBI GeneID: 21924
• NCBI Accession #: NP_033419.1
• NCBI GenBank Nucleotide #: NM_009393.2
• UniProt Accession #: P19123
• Molecular Weight: 18,421 Da
• NCBI Official Full Name: troponin C, slow skeletal and cardiac muscles
• NCBI Official Synonym Full Names: troponin C, cardiac/slow skeletal
• NCBI Official Symbol: Tnnc1
• NCBI Official Synonym Symbols: TnC; cTnC; cTnI; tncc; AI874626
• NCBI Protein Information: troponin C, slow skeletal and cardiac muscles; TN-C
• UniProt Protein Name: Troponin C, slow skeletal and cardiac muscles
• Protein Family: Troponin
• UniProt Gene Name: Tnnc1
• UniProt Synonym Gene Names: Tncc; TN-C
• UniProt Entry Name: TNNC1_MOUSE

Uniprot Description

TNNC1: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Defects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in TNNC1 are the cause of familial hypertrophic cardiomyopathy type 13 (CMH13). A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the troponin C family.

Protein type: Calcium-binding; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; troponin complex; contractile fiber; mitochondrion; actin cytoskeleton

Molecular Function: actin filament binding; troponin T binding; protein homodimerization activity; troponin I binding; metal ion binding; calcium ion binding; calcium-dependent protein binding

Biological Process: regulation of ATPase activity; response to metal ion; transition between fast and slow fiber; regulation of muscle contraction; diaphragm contraction; regulation of muscle filament sliding speed; ventricular cardiac muscle morphogenesis; cardiac muscle contraction