Rabbit anti-Human TRIP13 Polyclonal Antibody | anti-TRIP13 antibody

TRIP13, NT (Thyroid Receptor-interacting Protein 13, Thyroid Hormone Receptor Interactor 13, TR-interacting Protein 13, TRIP-13, Human Papillomavirus Type 16 E1 Protein-binding Protein, HPV16 E1 Protein-binding Protein, 16E1-BP) (Biotin)

Cat. Num. AAA6502581

• Gene Names: TRIP13; MVA3; 16E1BP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: TRIP13; Polyclonal Antibody; NT (Thyroid Receptor-interacting Protein 13; Thyroid Hormone Receptor Interactor 13; TR-interacting Protein 13; TRIP-13; Human Papillomavirus Type 16 E1 Protein-binding Protein; HPV16 E1 Protein-binding Protein; 16E1-BP) (Biotin); anti-TRIP13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRIP13.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TRIP13 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human TRIP13 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TRIP13 Antibody (N-term) in K562, Hela, 293 cell line lysates (35ug/lane). TRIP13 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with NRP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TRIP13 Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of TRIP13 (arrow) using rabbit polyclonal TRIP13 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TRIP13 gene.)

Product Categories/Family for anti-TRIP13 antibody

Antibodies; Binding Proteins

References

1. Rush, J., Nat. Biotechnol. 23(1), 94-101 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 11321607
• NCBI GeneID: 9319
• NCBI Accession #: NP_004228
• NCBI GenBank Nucleotide #: NM_004237
• UniProt Accession #: Q15645
• Molecular Weight: 49kDa
• NCBI Official Full Name: pachytene checkpoint protein 2 homolog isoform 1
• NCBI Official Synonym Full Names: thyroid hormone receptor interactor 13
• NCBI Official Symbol: TRIP13
• NCBI Official Synonym Symbols: MVA3; 16E1BP
• NCBI Protein Information: pachytene checkpoint protein 2 homolog
• UniProt Protein Name: Pachytene checkpoint protein 2 homolog
• UniProt Gene Name: TRIP13
• UniProt Synonym Gene Names: PCH2; 16E1-BP; HPV16 E1 protein-binding protein; TR-interacting protein 13; TRIP-13
• UniProt Entry Name: PCH2_HUMAN

NCBI Description

This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer. [provided by RefSeq, Oct 2009]

Uniprot Description

TRIP13: Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways. Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double- strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes. Belongs to the AAA ATPase family. PCH2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: male germ cell nucleus; nucleus

Molecular Function: identical protein binding; protein binding; transcription cofactor activity; ATP binding

Biological Process: oogenesis; transcription from RNA polymerase II promoter; synaptonemal complex assembly; male meiosis I; meiotic recombination; double-strand break repair; spermatogenesis; oocyte maturation; spermatid development; female meiosis I