Rabbit anti-Human TRIM65 Polyclonal Antibody | anti-TRIM65 antibody

TRIM65, ID (TRIM65, Tripartite motif-containing protein 65) (Azide free) (HRP)

Cat. Num. AAA6358554

• Gene Names: TRIM65; 4732463G12Rik
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRIM65; Polyclonal Antibody; ID (TRIM65; Tripartite motif-containing protein 65) (Azide free) (HRP); Tripartite motif-containing protein 65; anti-TRIM65 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 517

• Applicable Applications for anti-TRIM65 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TRIM65 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 334-364 amino acids from the Central region of human TRIM65.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TRIM65 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TRIM65 antibody detected the TRIM65 protein (arrow).)

Immunohistochemistry (IHC)

(TRIM65 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM65 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of TRIM65 Antibody (Center) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(TRIM65 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TRIM65 antibody

TRIM65 belongs to the TRIM/RBCC family. It contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger.

Product Categories/Family for anti-TRIM65 antibody

Antibodies; Transcription Factors; TRIM (Tripartite Motif)

References

Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 38679905
• NCBI GeneID: 201292
• NCBI Accession #: NP_775818.2
• NCBI GenBank Nucleotide #: NP_775818.2
• UniProt Accession #: Q6PJ69
• NCBI Official Full Name: tripartite motif-containing protein 65 isoform 1
• NCBI Official Synonym Full Names: tripartite motif containing 65
• NCBI Official Symbol: TRIM65
• NCBI Official Synonym Symbols: 4732463G12Rik
• NCBI Protein Information: tripartite motif-containing protein 65
• UniProt Protein Name: Tripartite motif-containing protein 65
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM65
• UniProt Entry Name: TRI65_HUMAN