Goat anti-Human TRIM5 Polyclonal Antibody | anti-TRIM5 antibody

TRIM5 (RNF88, Tripartite Motif-containing Protein 5, RING Finger Protein 88, RNF88) (FITC)

Cat. Num. AAA6503597

• Gene Names: TRIM5; RNF88; TRIM5alpha
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: TRIM5; Polyclonal Antibody; TRIM5 (RNF88; Tripartite Motif-containing Protein 5; RING Finger Protein 88; RNF88) (FITC); anti-TRIM5 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRIM5. Recognizes all 3 human isoforms.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 347

• Applicable Applications for anti-TRIM5 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to ASGILVNVKEEVTC, from human TRIM5, at N-terminal (NP_149023.1, NP_149083.1, NP_149084.1).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western Blot staining of Human Placenta lysate (35ug protein in RIPA buffer) using T8464-98N (2ug/ml) Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-TRIM5 antibody

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Five alternatively spliced transcript variants for this gene have been described. However, the full length nature of two of the variants has not been determined.

Product Categories/Family for anti-TRIM5 antibody

Antibodies; Transcription Factors; TRIM (Tripartite Motif)

References

1. Yap MW, Lindemann D, Stanke N, Reh J, Westphal D, Hanenberg H, Ohkura S, Stoye JP. Restriction of foamy viruses by primate Trim5alpha. J Virol. 2008 Jun;82(11):5429-39. 2. Yap MW, Mortuza GB, Taylor IA, Stoye JP. The design of artificial retroviral restriction factors. Virology. 2007 Sep 1;365(2):302-14. 3. Ohkura S, Yap MW, Sheldon T, Stoye JP. All three variable regions of the TRIM5alpha B30.2 domain can contribute to the specificity of retrovirus restriction. J Virol. 2006 Sep;80(17):8554-65.

NCBI and Uniprot Product Information

• NCBI GI #: 15011944
• NCBI GeneID: 85363
• NCBI Accession #: NP_149083.1; NP_149084.1
• NCBI GenBank Nucleotide #: NP_149083.1
• NCBI Official Full Name: tripartite motif protein TRIM5 isoform gamma
• NCBI Official Synonym Full Names: tripartite motif containing 5
• NCBI Official Symbol: TRIM5
• NCBI Official Synonym Symbols: RNF88; TRIM5alpha
• NCBI Protein Information: tripartite motif-containing protein 5
• Protein Family: Tripartite motif-containing protein

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]