Western Blot (WB)
(Western blot analysis of TRIM35 using HuvEc whole cell lysates)
Rabbit anti-Human TRIM35 Polyclonal Antibody | anti-TRIM35 antibody
TRIM35 Antibody
Gene Names
TRIM35; HLS5; MAIR
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TRIM35; Polyclonal Antibody; TRIM35 Antibody; 0710005M05Rik; A430106H13Rik; AW046487; Hemopoietic lineage switch protein 5; HLS5; KIAA1098; Macrophage derived apoptosis inducing RBCC protein; MAIR; MGC17233; mKIAA1098; NC8; Protein MAIR; Protein Nc8; TRI35_HUMAN; Tripartite motif containing 35; Tripartite motif containing protein 35; Tripartite motif-containing protein 35; anti-TRIM35 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
TRIM35 antibody detects endogenous levels of total TRIM35
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
493
Applicable Applications for anti-TRIM35 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human TRIM35
Subcellular Location
Cytoplasm. Nucleus. Found predominantly in cytoplasm with a granular distribution. Found in punctuate nuclear bodies.
Predicted Cross Reactivity
Rabbit, Dog
Similarity
Rabbit (88%), Dog (88%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of TRIM35 using HuvEc whole cell lysates)
Western Blot (WB)
(Western blot analysis of TRIM35 using HuvEc whole cell lysates)
Related Product Information for anti-TRIM35 antibody
Function: Reduces FGFR1-dependent tyrosine phosphorylation of PKM, inhibiting PKM-dependent lactate production, glucose metabolism, and cell growth (PubMed:25263439). Involved in the cell death mechanism (By similarity).
Subunit Structure: Interacts with PKM isoform M2, but not isoform M1; this interaction may compete with that between PKM and FGFR1, and hence reduces FGFR1-dependent tyrosine phosphorylation of PKM.
Similarity: The RING finger domain and the coiled-coil region are required for the apoptosis-inducing activity. Belongs to the TRIM/RBCC family.
Subunit Structure: Interacts with PKM isoform M2, but not isoform M1; this interaction may compete with that between PKM and FGFR1, and hence reduces FGFR1-dependent tyrosine phosphorylation of PKM.
Similarity: The RING finger domain and the coiled-coil region are required for the apoptosis-inducing activity. Belongs to the TRIM/RBCC family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 57 kDa
Predicted: 57 kDa
Predicted: 57 kDa
NCBI Official Full Name
tripartite motif-containing protein 35 isoform 1
NCBI Official Synonym Full Names
tripartite motif containing 35
NCBI Official Symbol
TRIM35
NCBI Official Synonym Symbols
HLS5; MAIR
NCBI Protein Information
tripartite motif-containing protein 35
UniProt Protein Name
Tripartite motif-containing protein 35
Protein Family
UniProt Gene Name
TRIM35
UniProt Synonym Gene Names
HLS5; KIAA1098
NCBI Description
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
Reduces FGFR1-dependent tyrosine phosphorylation of PKM, inhibiting PKM-dependent lactate production, glucose metabolism, and cell growth (PubMed:25263439). Involved in the cell death mechanism ().
Research Articles on TRIM35
Similar Products
Product Notes
The TRIM35 trim35 (Catalog #AAA9609205) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM35 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM35 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the TRIM35 trim35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM35, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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