Rabbit anti-Human TRIM28 Polyclonal Antibody | anti-TRIM28 antibody

TRIM28, NT (TRIM28, KAP1, RNF96, TIF1B, Transcription intermediary factor 1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KRAB-interacting protein 1, Nuclear corepressor KAP-1, RING finger protein 96, Tripartite motif-containing protein

Cat. Num. AAA6358255

• Gene Names: TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRIM28; Polyclonal Antibody; NT (TRIM28; KAP1; RNF96; TIF1B; Transcription intermediary factor 1-beta; E3 SUMO-protein ligase TRIM28; KRAB-associated protein 1; KRAB-interacting protein 1; Nuclear corepressor KAP-1; RING finger protein 96; Tripartite motif-containing protein; Tripartite motif-containing protein 28; anti-TRIM28 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TRIM28 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TRIM28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-215 amino acids from the N-terminal region of human TRIM28.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TRIM28 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM28 antibody detected the TRIM28 protein (arrow).)

Immunohistochemistry (IHC)

(TRIM28 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM28 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with TRIM28 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly. )

Related Product Information for anti-TRIM28 antibody

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Product Categories/Family for anti-TRIM28 antibody

Antibodies; Transcription Factors; TRIM (Tripartite Motif)

References

Lai, I.L., et al. J. Biol. Chem. 285(10):7187-7196(2010). Yokoe, T., et al. Ann. Surg. Oncol. 17(3):821-828(2010). Noon, A.T., et al. Nat. Cell Biol. 12(2):177-184(2010). Li, X., et al. Sci Signal 3 (119), RA32 (2010): Groner, A.C., et al. PLoS Genet. 6 (3), E1000869 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 14971416
• NCBI GeneID: 10155
• NCBI Accession #: NP_005753.1
• NCBI GenBank Nucleotide #: NM_005762
• UniProt Accession #: Q13263; O00677; Q7Z632; Q93040; Q96IM1
• Molecular Weight: 79,474 Da
• NCBI Official Full Name: Homo sapiens tripartite motif containing 28 (TRIM28), mRNA
• NCBI Official Synonym Full Names: tripartite motif containing 28
• NCBI Official Symbol: TRIM28
• NCBI Official Synonym Symbols: KAP1; TF1B; RNF96; TIF1B; PPP1R157
• NCBI Protein Information: transcription intermediary factor 1-beta; E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; RING finger protein 96; TIF1-beta; nuclear corepresso
• UniProt Protein Name: Transcription intermediary factor 1-beta
• Protein Family: Transcription intermediary factor
• UniProt Gene Name: TRIM28
• UniProt Synonym Gene Names: KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1
• UniProt Entry Name: TIF1B_HUMAN

NCBI Description

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Uniprot Description

TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Protein type: EC 6.3.2.-; Ligase; Protein kinase, atypical; Ubiquitin conjugating system; Kinase, protein; Ubiquitin ligase; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: nucleoplasm; nuclear heterochromatin; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; transcription factor activity; transcription corepressor activity; ligase activity; protein kinase activity

Biological Process: transcription initiation from RNA polymerase II promoter; convergent extension involved in axis elongation; positive regulation of DNA repair; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein oligomerization; protein sumoylation; positive regulation of transcription factor import into nucleus; innate immune response; epithelial to mesenchymal transition; gene expression; negative regulation of transcription, DNA-dependent; DNA methylation during embryonic development; embryo implantation