Rabbit TRIM27 Polyclonal Antibody | anti-TRIM27 antibody

TRIM27 Antibody

Cat. Num. AAA9611533

• Gene Names: TRIM27; RFP; RNF76
• Reactivity: Human, Mouse, Rat, Monkey; Predicted: Pig, Bovine, Horse, Sheep, Dog
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: TRIM27; Polyclonal Antibody; TRIM27 Antibody; AW538890; DAAP-182E11.3; MGC189472; OTTHUMP00000029314; Ret finger protein; RFP; RFP transforming protein; Rfp1; RING finger protein 76; RNF76; TRI27_HUMAN; TRIM 27; Trim27; Tripartite motif containing 27; Tripartite motif containing protein 27; Tripartite motif protein TRIM27; Tripartite motif-containing protein 27; Zinc finger protein RFP; anti-TRIM27 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey; Predicted: Pig, Bovine, Horse, Sheep, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TRIM27 Antibody detects endogenous levels of TRIM27.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 513

• Applicable Applications for anti-TRIM27 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human TRIM27.
• Tissue Specificity: Expressed in testis namely within the seminiferous tubules.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Endosome; Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, using TRIM27 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from COS-7, using TRIM27 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-TRIM27 antibody

Description: E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.

Subunit Structure: Homomultimerizes. Interacts with PML, EIF3S6, EPC1, CHD4 and EID1. Interacts with MAGED4, MAGEF1 and MAGEL2. Interacts with herpes simplex virus protein ICP0.

Similarity: The coiled-coil region mediates interaction with EPC1 and CHD4. The B box and coiled-coil domains mediate interaction with PML. The B box and the distal coiled-coil domains mediate homomultimerisation. The B30.2 domain mediates interaction with EIF3S6. Belongs to the TRIM/RBCC family.

NCBI and Uniprot Product Information

• NCBI GI #: 5730009
• NCBI GeneID: 5987
• NCBI Accession #: NP_006501
• NCBI GenBank Nucleotide #: NP_006501.1
• UniProt Accession #: P14373
• Molecular Weight: Observed: 58 kDa; Predicted: 59 kDa
• NCBI Official Full Name: zinc finger protein RFP
• NCBI Official Synonym Full Names: tripartite motif containing 27
• NCBI Official Symbol: TRIM27
• NCBI Official Synonym Symbols: RFP; RNF76
• NCBI Protein Information: zinc finger protein RFP
• UniProt Protein Name: Zinc finger protein RFP
• Protein Family: Zinc finger protein
• UniProt Gene Name: TRIM27
• UniProt Synonym Gene Names: RFP; RNF76
• UniProt Entry Name: TRI27_HUMAN

NCBI Description

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]