Western Blot (WB)
(Western BlotPositive WB detected in: Raji whole cell lysateAll lanes: TRIB1 antibody at 4.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42, 24 KDaObserved band size: 42 KDa)
Rabbit anti-Human TRIB1 Polyclonal Antibody | anti-TRIB1 antibody
TRIB1 Antibody
IF: 1:50-1:200
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Western Blot (WB)
(Western BlotPositive WB detected in: Raji whole cell lysateAll lanes: TRIB1 antibody at 4.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42, 24 KDaObserved band size: 42 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: Raji whole cell lysateAll lanes: TRIB1 antibody at 4.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42, 24 KDaObserved band size: 42 KDa)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with MBS7107443 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with MBS7107443 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
NCBI and Uniprot Product Information
Uniprot Description
Adapter protein involved in protein degradation by interacting with RFWD2/COP1 ubiquitin ligase (PubMed:27041596). The RFWD2-binding motif is masked by autoinhibitory interactions with the protein kinase domain (PubMed:26455797). Serves to alter RFWD2 substrate specificity by directing the activity of RFWD2 toward CEBPA (PubMed:27041596). Binds selectively the recognition sequence of CEBPA (PubMed:26455797). Regulates myeloid cell differentiation by altering the expression of CEBPA in a RFWD2-dependent manner (). Controls macrophage, eosinophil and neutrophil differentiation via the COP1-binding domain (). Interacts with MAPK kinases and regulates activation of MAP kinases, but has no kinase activity (PubMed:15299019, PubMed:26455797).
Research Articles on TRIB1
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Product Notes
The TRIB1 trib1 (Catalog #AAA7107443) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIB1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:5000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the TRIB1 trib1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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