Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using TRI18 antibody.)
Rabbit TRI18 Polyclonal Antibody | anti-TRI18 antibody
TRI18 Antibody
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using TRI18 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using TRI18 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using TRI18 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using TRI18 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using TRI18 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using TRI18 antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Multiple different transcript variants are generated by alternate splicing; however, the full-length nature of some of the variants has not been determined. [provided by RefSeq, Jul 2010]
Uniprot Description
MID1: Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination. Defects in MID1 are the cause of Opitz GBBB syndrome 1 (OGS1). A congenital midline malformation syndrome characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate- laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. MID1 mutations produce proteins with a decreased affinity for microtubules. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.-; Cytoskeletal; Ubiquitin ligase; EC 6.3.2.19; Ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: Xp22
Cellular Component: microtubule; microtubule associated complex; cytoplasmic microtubule; spindle; cytosol
Molecular Function: protein homodimerization activity; zinc ion binding; protein heterodimerization activity; microtubule binding; ubiquitin protein ligase binding; phosphoprotein binding; ligase activity
Biological Process: negative regulation of microtubule depolymerization; metabolic process; cytokine and chemokine mediated signaling pathway; microtubule cytoskeleton organization and biogenesis; pattern specification process; positive regulation of stress-activated MAPK cascade
Disease: Tracheoesophageal Fistula With Or Without Esophageal Atresia; Opitz Gbbb Syndrome, X-linked
Research Articles on TRI18
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Product Notes
The TRI18 mid1 (Catalog #AAA9411108) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRI18 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRI18 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500~1:3000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:500. Researchers should empirically determine the suitability of the TRI18 mid1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRI18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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