Human Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit | TREM2 elisa kit

Human Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit

Cat. Num. AAA2022102

• Gene Names: TREM2; TREM-2; Trem2a; Trem2b; Trem2c
• Reactivity: Human
• Synonyms: Triggering Receptor Expressed On Myeloid Cells 2 (TREM2); Human Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit; Trem2a; Trem2b; Trem2c; Triggering receptor expressed on monocytes 2; TREM2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TREM2. No significant cross-reactivity or interference between TREM2 and analogues was observed.
• Sequence Length: 222

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5-4,000pg/mL
• Sensitivity: < 26.2pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TREM2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TREM2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

()

Related Product Information for TREM2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TREM2 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TREM2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TREM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TREM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TREM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TREM2 elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 9507203
• NCBI GeneID: 54209
• NCBI Accession #: NP_061838.1
• NCBI GenBank Nucleotide #: NM_018965.3
• UniProt Accession #: Q9NZC2; Q8N5H8; Q8WYN6
• Molecular Weight: 24,669 Da
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 2 isoform 1
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 2
• NCBI Official Symbol: TREM2
• NCBI Official Synonym Symbols: TREM-2; Trem2a; Trem2b; Trem2c
• NCBI Protein Information: triggering receptor expressed on myeloid cells 2
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 2
• Protein Family: Triggering receptor expressed on myeloid cells
• UniProt Gene Name: TREM2
• UniProt Synonym Gene Names: TREM-2
• UniProt Entry Name: TREM2_HUMAN

NCBI Description

This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]

Uniprot Description

TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: intracellular membrane-bound organelle; plasma membrane; extracellular region; integral to membrane

Molecular Function: peptidoglycan binding; lipopolysaccharide binding; receptor activity

Biological Process: axon guidance; positive regulation of peptidyl-tyrosine phosphorylation; detection of lipopolysaccharide; detection of peptidoglycan; innate immune response; positive regulation of calcium-mediated signaling; humoral immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II

Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy