Rabbit anti-Human TREM1 Polyclonal Antibody | anti-TREM1 antibody

TREM1, CT (TREM1, Triggering receptor expressed on myeloid cells 1, Triggering receptor expressed on monocytes 1, CD354) (AP)

Cat. Num. AAA6358033

• Gene Names: TREM1; CD354; TREM-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TREM1; Polyclonal Antibody; CT (TREM1; Triggering receptor expressed on myeloid cells 1; Triggering receptor expressed on monocytes 1; CD354) (AP); CD354; anti-TREM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TREM1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TREM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 161-191 amino acids selected from the C-terminal region of human TREM1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TREM1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TREM1 antibody detected the TREM1 protein (arrow).)

Related Product Information for anti-TREM1 antibody

Monocyte/macrophage-and neutrophil-mediated inflammatory responses can be stimulated through a variety of receptors, including G protein-linked 7-transmembrane receptors (e.g., FPR1; MIM 136537), Fc receptors (see MIM 146790), CD14 (MIM 158120) and Toll-like receptors (e.g., TLR4; MIM 603030), and cytokine receptors (e.g., IFNGR1; MIM 107470). Engagement of these receptors can also prime myeloid cells to respond to other stimuli. Myeloid cells express receptors belonging to the Ig superfamily, such as TREM1, or to the C-type lectin superfamily. Depending on their transmembrane and cytoplasmic sequence structure, these receptors have either activating (e.g., KIR2DS1; MIM 604952) or inhibitory functions (e.g., KIR2DL1; MIM 604936).

Product Categories/Family for anti-TREM1 antibody

Antibodies; CD Markers

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Tomita, H., et al. J. Rheumatol. 37(4):787-791(2010). Haselmayer, P., et al. J Innate Immun 1(6):582-591(2009). Kim, J., et al. Clin. Exp. Rheumatol. 27(5):773-778(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 8924262
• NCBI GeneID: 54210
• NCBI Accession #: NP_061113.1
• NCBI GenBank Nucleotide #: NM_018643.3
• UniProt Accession #: Q9NP99; Q53FL8; Q5T2C9; Q86YU1; B4DWG2; K7EJW1
• Molecular Weight: 25,574 Da
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 1 isoform 1
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 1
• NCBI Official Symbol: TREM1
• NCBI Official Synonym Symbols: CD354; TREM-1
• NCBI Protein Information: triggering receptor expressed on myeloid cells 1
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 1
• Protein Family: Triggering receptor expressed on myeloid cells
• UniProt Gene Name: TREM1
• UniProt Synonym Gene Names: TREM-1
• UniProt Entry Name: TREM1_HUMAN

NCBI Description

This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

TREM1: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: extracellular region; integral to membrane; plasma membrane

Molecular Function: receptor activity

Biological Process: neutrophil chemotaxis; innate immune response; cytokine secretion during immune response; blood coagulation; leukocyte migration; chemokine metabolic process; humoral immune response