Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS649623. TREF1 (arrow) was detected usingMBS649623.)
Rabbit anti-Human TREF1 Polyclonal Antibody | anti-TREF1 antibody
TREF1, CT (TRERF1, BCAR2, RAPA, TREP132, Transcriptional-regulating factor 1, Breast cancer anti-estrogen resistance 2, Transcriptional-regulating protein 132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132) (Biotin)
Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS649623. TREF1 (arrow) was detected usingMBS649623.)
Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS649623. TREF1 (arrow) was detected usingMBS649623.)
Immunofluorescence (IF)
(Fluorescent image of MCF-7 cell stained withMBS649623. MCF-7 cells were fixed with 4% PFA (20mins), permeabilized with Triton X-100 (0.1%, 10mins), then incubated with MBS649623 (1:25, 1hr at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50mins at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).TREF1 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of MCF-7 cell stained withMBS649623. MCF-7 cells were fixed with 4% PFA (20mins), permeabilized with Triton X-100 (0.1%, 10mins), then incubated with MBS649623 (1:25, 1hr at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50mins at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).TREF1 immunoreactivity is localized to Nucleus significantly.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of K562 cells MBS649623 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of K562 cells MBS649623 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1. [provided by RefSeq, Jul 2008]
Uniprot Description
TReP-132: Binds DNA and activates transcription of CYP11A1. Interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1
Protein type: C2H2-type zinc finger protein; Transcription factor; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 6p21.1-p12.1
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: ligand-dependent nuclear receptor transcription coactivator activity; metal ion binding; chromatin binding; DNA bending activity; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; homeostatic process; positive regulation of transcription, DNA-dependent; multicellular organismal development; cholesterol catabolic process; regulation of hormone biosynthetic process; steroid biosynthetic process
Research Articles on TREF1
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Product Notes
The TREF1 trerf1 (Catalog #AAA6358024) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TREF1, CT (TRERF1, BCAR2, RAPA, TREP132, Transcriptional-regulating factor 1, Breast cancer anti-estrogen resistance 2, Transcriptional-regulating protein 132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TREF1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TREF1 trerf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TREF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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