Rabbit anti-Human, Mouse TRBP1 Polyclonal Antibody | anti-TRBP1 antibody

TRBP1 Antibody

Cat. Num. AAA9408435

• Gene Names: TARBP1; TRM3; TRP185; TRP-185
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column
• Synonyms: TRBP1; Polyclonal Antibody; TRBP1 Antibody; TAR RNA binding protein 1; TARBP1; TRP-185; TRP-1; anti-TRBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 1621

• Applicable Applications for anti-TRBP1 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 17 amino acid peptide from near the center of human TRBP1.
• Target Name: TRBP1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TRBP1 in 3T3 cell lysate with TRBP1 antibody at (A) 1 and (B) 2 ug/mL.)

Related Product Information for anti-TRBP1 antibody

The human trans-activation response (TAR) RNA binding protein 1 (TRBP1) was initially identified as a protein that binds to the HIV-1 TAR RNA and activates the long terminal repeat (LTR) expression in the absence and presence of the viral trans-activator Tat. This binding is enhanced by the presence of co-factors such as elongation factor 1a (EF-1a), polypyrimidine tract-binding protein (PTB), and the chaperonin-like protein stimulator of TAR RNA-binding proteins (SRB). TRBP1 may act to disengage RNA polymerase II from TAR during transcriptional elongation if the RNA polymerase stalls at the TAR during transcriptional elongation.

Product Categories/Family for anti-TRBP1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 110825988
• NCBI GeneID: 6894
• NCBI Accession #: NP_005637
• NCBI GenBank Nucleotide #: NM_005646.3
• Molecular Weight: 181,675 Da
• NCBI Official Full Name: probable methyltransferase TARBP1
• NCBI Official Synonym Full Names: TAR (HIV-1) RNA binding protein 1
• NCBI Official Symbol: TARBP1
• NCBI Official Synonym Symbols: TRM3; TRP185; TRP-185
• NCBI Protein Information: probable methyltransferase TARBP1
• UniProt Protein Name: Probable methyltransferase TARBP1
• UniProt Gene Name: TARBP1
• UniProt Synonym Gene Names: TRM3; TRP185; TRP-185
• UniProt Entry Name: TARB1_HUMAN

NCBI Description

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. This element forms a stable stem-loop structure and can be bound by either the protein encoded by this gene or by RNA polymerase II. This protein may act to disengage RNA polymerase II from TAR during transcriptional elongation. Alternatively spliced transcripts of this gene may exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TARBP1: Probable S-adenosyl-L-methionine-dependent methyltransferase which methylates RNA molecules such as tRNAs. In case of infection by HIV-1, it binds to the loop region of TAR RNA, a region also bound by RNA polymerase II. Binding of TARBP1 and RNA polymerase II to HIV-1 TAR RNA is mutually exclusive, suggesting that TARBP1 may function alone or in conjunction with HIV-1 Tat to disengage RNA polymerase II from HIV-1 TAR RNA. May act by methylating HIV-1 TAR RNA. Belongs to the RNA methyltransferase TrmH family.

Protein type: Methyltransferase; EC 2.1.1.-; RNA-binding

Chromosomal Location of Human Ortholog: 1q42.3

Cellular Component: nucleus

Molecular Function: RNA binding; RNA methyltransferase activity

Biological Process: RNA processing; regulation of transcription from RNA polymerase II promoter; RNA methylation