Western Blot (WB)
(TGM2 Antibody (Center K444) western blot analysis in HUVEC,K562 cell line lysates (35ug/lane).This demonstrates the TGM2 antibody detected the TGM2 protein (arrow).)
Rabbit anti-Human Transglutaminase Polyclonal Antibody | anti-TGM2 antibody
Transglutaminase, ID (K444) (Protein-glutamine gamma-glutamyltransferase 2, Tissue Transglutaminase, Transglutaminase C, TGase C, TGC, TG(C), Transglutaminase-2, TGase-2, Transglutaminase H, TGase-H, TGM2) (PE)
FLISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Western Blot (WB)
(TGM2 Antibody (Center K444) western blot analysis in HUVEC,K562 cell line lysates (35ug/lane).This demonstrates the TGM2 antibody detected the TGM2 protein (arrow).)
Western Blot (WB)
(TGM2 Antibody (Center K444) western blot analysis in HUVEC,K562 cell line lysates (35ug/lane).This demonstrates the TGM2 antibody detected the TGM2 protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of U251 cell stained with TGM2 Antibody (Center K444).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TGM2 immunoreactivity is localized to Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of U251 cell stained with TGM2 Antibody (Center K444).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TGM2 immunoreactivity is localized to Cytoplasm significantly.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. . Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described.
Protein type: Transferase; EC 2.3.2.13
Chromosomal Location of Human Ortholog: 20q12
Cellular Component: focal adhesion; mitochondrion; plasma membrane; cytosol
Molecular Function: protein domain specific binding; protein binding; GTP binding; protein-glutamine gamma-glutamyltransferase activity; metal ion binding
Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of cell adhesion; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; positive regulation of apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of smooth muscle cell proliferation; blood vessel remodeling; protein homooligomerization; apoptotic cell clearance; elevation of mitochondrial calcium ion concentration; positive regulation of inflammatory response
Research Articles on TGM2
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Product Notes
The TGM2 tgm2 (Catalog #AAA6503442) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Transglutaminase, ID (K444) (Protein-glutamine gamma-glutamyltransferase 2, Tissue Transglutaminase, Transglutaminase C, TGase C, TGC, TG(C), Transglutaminase-2, TGase-2, Transglutaminase H, TGase-H, TGM2) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Transglutaminase can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-1:50 FLISA: 1:1,000 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TGM2 tgm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transglutaminase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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