Rabbit Transcription factor p65 Polyclonal Antibody | anti-RELA antibody

Rabbit anti-human Transcription factor p65 polyclonal Antibody

Cat. Num. AAA1489473

• Gene Names: RELA; p65; NFKB3
• Reactivity: Human. Other species are not tested.
• Applications: ELISA, Western Blot, Immunohistochemistry, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Synonyms: Transcription factor p65; Polyclonal Antibody; Rabbit anti-human Transcription factor p65 polyclonal Antibody; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; RELA; NFKB3; anti-RELA antibody

• Host: Rabbit
• Reactivity: Human. Other species are not tested.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Sequence Length: 548

• Applicable Applications for anti-RELA antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Transcription factor p65 protein
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-109 / sc-7151 / sc-372
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: 293 whole cell lysate, NIH/3T3 whole cell lysateAll lanes: RELA antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61,59,60 KDaObserved band size: 61 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS1489473 diluted at 1:600 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(IHC image of MBS1489473 diluted at 1:600 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunofluorescence (IF)

(Immunofluorescence staining of PC3 cells with MBS1489473 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Chromatin Immunoprecipitation (ChIP)

(Chromatin Immunoprecipitation Hela(1.2^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-NF-kB p65 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the IkBα promoter.)

Related Product Information for anti-RELA antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

NCBI and Uniprot Product Information

• NCBI GI #: 223468681
• NCBI GeneID: 5970
• NCBI Accession #: NP_001138610.1
• NCBI GenBank Nucleotide #: NM_001145138.1
• UniProt Accession #: Q04206; Q6GTV1; Q6SLK1
• Molecular Weight: 59,910 Da
• NCBI Official Full Name: transcription factor p65 isoform 2
• NCBI Official Synonym Full Names: v-rel avian reticuloendotheliosis viral oncogene homolog A
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65
• UniProt Protein Name: Transcription factor p65
• Protein Family: Transcription factor
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN

NCBI Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Transcription factor; DNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; transcription factor complex

Molecular Function: actinin binding; chromatin binding; chromatin DNA binding; DNA binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein complex binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: acetaldehyde metabolic process; activation of NF-kappaB transcription factor; aging; cellular defense response; cytokine and chemokine mediated signaling pathway; defense response to virus; hair follicle development; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; liver development; membrane protein intracellular domain proteolysis; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of insulin receptor signaling pathway; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; organ morphogenesis; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interleukin-12 biosynthetic process; positive regulation of Schwann cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; response to amino acid stimulus; response to cAMP; response to cobalamin; response to drug; response to insulin stimulus; response to morphine; response to muramyl dipeptide; response to organic substance; response to progesterone stimulus; response to UV-B; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter; viral reproduction