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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human Trans Golgi Network Protein 2 Polyclonal Antibody | anti-TGOLN2 antibody

Polyclonal Antibody to Trans Golgi Network Protein 2 (TGOLN2)

Gene Names
TGOLN2; TGN38; TGN46; TGN48; TGN51; TTGN2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Trans Golgi Network Protein 2; Polyclonal Antibody; Polyclonal Antibody to Trans Golgi Network Protein 2 (TGOLN2); TGN51; TGN46; TGN48; TGN38; TTGN2; Trans-Golgi Network Protein; anti-TGOLN2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TGOLN2. It has been selected for its ability to recognize TGOLN2 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-TGOLN2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Fragment
TGOLN2 (Ala22~Glu323)
Conjugate
No Conjugate
Cross Reactivity
Human
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant TGOLN2 (Ala22~Glu323) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2079125
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded kidney tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,541 Da
NCBI Official Full Name
trans-Golgi network integral membrane protein 2 isoform 2
NCBI Official Synonym Full Names
trans-golgi network protein 2
NCBI Official Symbol
TGOLN2
NCBI Official Synonym Symbols
TGN38; TGN46; TGN48; TGN51; TTGN2
NCBI Protein Information
trans-Golgi network integral membrane protein 2
UniProt Protein Name
Trans-Golgi network integral membrane protein 2
UniProt Gene Name
TGOLN2
UniProt Synonym Gene Names
TGN46; TGN51

NCBI Description

This gene encodes a type I integral membrane protein that is localized to the trans-Golgi network, a major sorting station for secretory and membrane proteins. The encoded protein cycles between early endosomes and the trans-Golgi network, and may play a role in exocytic vesicle formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

TGOLN2: May be involved in regulating membrane traffic to and from trans-Golgi network. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: clathrin-coated vesicle membrane; cytosol; endoplasmic reticulum lumen; endosome; Golgi apparatus; integral component of membrane; nucleoplasm; plasma membrane; trans-Golgi network; trans-Golgi network transport vesicle; transport vesicle

Molecular Function: protein binding

Biological Process: cellular protein metabolic process; Golgi to endosome transport; post-translational protein modification

Research Articles on TGOLN2

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Product Notes

The TGOLN2 tgoln2 (Catalog #AAA2033791) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Trans Golgi Network Protein 2 (TGOLN2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Trans Golgi Network Protein 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the TGOLN2 tgoln2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Trans Golgi Network Protein 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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