Rabbit anti-Human KRT12 Polyclonal Antibody | anti-KRT12 antibody

KRT12, CT (KRT12, Keratin, type I cytoskeletal 12, Cytokeratin-12, Keratin-12) (Azide free) (HRP)

Cat. Num. AAA6314550

• Gene Names: KRT12; K12
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: KRT12; Polyclonal Antibody; CT (KRT12; Keratin; type I cytoskeletal 12; Cytokeratin-12; Keratin-12) (Azide free) (HRP); Keratin-12; anti-KRT12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KRT12.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-KRT12 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa442-471 in the C-terminal region of human KRT12, Swiss/UniProt Accession: Q99456
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS649883.This demonstrates that MBS649883 detected the KRT12 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma using MBS649883 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS649883 for immunohistochemistry.)

Testing Data

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Product Categories/Family for anti-KRT12 antibody

Antibodies; Cytokeratin

References

1. Clausen, I., et al. Mol. Vis. 16, 954-960 (2010). 2. Seto, T., et al. Jpn. J. Ophthalmol. 52(3):224-226(2008). 3. Nielsen, K., et al. Cornea 27(1):100-102(2008). 4. Sullivan, L.S., et al. Mol. Vis. 13, 975-980 (2007). 5. Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4557699
• NCBI GeneID: 3859
• NCBI Accession #: NP_000214.1
• NCBI GenBank Nucleotide #: NM_000223.3
• UniProt Accession #: Q99456; B2R9E0
• Molecular Weight: 53,511 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 12
• NCBI Official Synonym Full Names: keratin 12
• NCBI Official Symbol: KRT12
• NCBI Official Synonym Symbols: K12
• NCBI Protein Information: keratin, type I cytoskeletal 12; CK-12; keratin-12; cytokeratin-12
• UniProt Protein Name: Keratin, type I cytoskeletal 12
• Protein Family: Keratin
• UniProt Gene Name: KRT12
• UniProt Synonym Gene Names: CK-12; K12
• UniProt Entry Name: K1C12_HUMAN

Uniprot Description

Function: May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity

By similarity.

Subunit structure: Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.

Tissue specificity: Cornea specific. Ref.1

Involvement in disease: Corneal dystrophy, Meesmann (MECD) [MIM:122100]: An autosomal dominant corneal disease characterized by fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2 Ref.3 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.