Rabbit anti-Human TRAM2 Polyclonal Antibody | anti-TRAM2 antibody

TRAM2, CT (TRAM2, KIAA0057, Translocating chain-associated membrane protein 2) (FITC)

Cat. Num. AAA6357948

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRAM2; Polyclonal Antibody; CT (TRAM2; KIAA0057; Translocating chain-associated membrane protein 2) (FITC); Translocating chain-associated membrane protein 2; anti-TRAM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TRAM2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TRAM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349-378 amino acids from the C-terminal region of human TRAM2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TRAM2 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TRAM2 antibody detected the TRAM2 protein (arrow).)

Immunohistochemistry (IHC)

(TRAM2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAM2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-TRAM2 antibody

Antibodies; KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 6912450
• NCBI GeneID: 9697
• NCBI Accession #: NP_036420.1
• NCBI GenBank Nucleotide #: NM_012288.3
• UniProt Accession #: Q15035; A8K6T6
• Molecular Weight: 43kDa
• NCBI Official Full Name: translocating chain-associated membrane protein 2
• NCBI Official Synonym Full Names: translocation associated membrane protein 2
• NCBI Official Symbol: TRAM2
• NCBI Protein Information: translocating chain-associated membrane protein 2
• UniProt Protein Name: Translocating chain-associated membrane protein 2
• Protein Family: Translocating chain-associated membrane protein
• UniProt Gene Name: TRAM2
• UniProt Synonym Gene Names: KIAA0057

NCBI Description

TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum (ER) membrane.[supplied by OMIM, Jul 2004]

Uniprot Description

Necessary for collagen type I synthesis. May couple the activity of the ER Ca2+ pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca2+ concentration at the site of collagen synthesis, and a high Ca2+ concentration may be necessary for the function of molecular chaperones involved in collagen folding. Required for proper insertion of the first transmembrane helix N-terminus of TM4SF20 into the ER lumen, may act as a ceramide sensor for regulated alternative translocation (RAT) (PubMed:27499293).