Rabbit anti-Human, Pig TRalpha Polyclonal Antibody | anti-TRalpha antibody

TRalpha polyclonal antibody

Cat. Num. AAA3015840

• Gene Names: THRA; AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
• Reactivity: Human, Pig
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Synonyms: TRalpha; Polyclonal Antibody; TRalpha polyclonal antibody; Thyroid hormone receptor alpha; Nuclear receptor subfamily 1 group A member 1; V-erbA-related protein 7; EAR-7; c-erbA-1; c-erbA-alpha; anti-TRalpha antibody

• Host: Rabbit
• Reactivity: Human, Pig
• Clonality: Polyclonal
• Specificity: TRalpha polyclonal antibody detects endogenous levels of TRalpha protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-TRalpha antibody: Western Blot (WB)
• Application Notes: WB: 1:2000~1:5000
• Immunogen: Synthetic peptide, corresponding to Human TRalpha.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of TR? polyclonal antibody at 1:5000 dilution Lane1:THP-1 whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:The Kidney tissue lysate of Pig(40ug) Lane4:The Liver tissue lysate of Pig(40ug))

Related Product Information for anti-TRalpha antibody

Thyroid hormone nuclear receptors (TRs) are ligand-dependent transcription factors which regulate and control many metabolic and developmental processes. There are two genes encoding TRs identified to date, TRalpha and TRbeta. TRs bind to thyroid hormone response elements (TREs) with half-site binding motifs in the orientation of palindromes, direct repeats or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3'-triiodo-L-thyronine (T3). Transcriptional regulation by TRs is also modulated by heterodimerization with TR nuclear accessory proteins, the most extensively characterized of which are the retinoid X receptors (RXRalpha, RXRbeta and RXRgamma). The TRalpha isoform TRalpha1 can display both a nuclear and undefined cytoplasmic location, and is the only TR that is imported into the mitochondrial matrix. TRalpha2 is a C-terminal variant of TRalpha1 that does not bind thyroid hormones (THs) and weakly binds DNA. TRalpha2 acts as a dominant negative antagonist of TH signalling.

NCBI and Uniprot Product Information

• NCBI GI #: 300116307
• NCBI GeneID: 7067
• NCBI Accession #: NP_001177847.1
• NCBI GenBank Nucleotide #: NM_001190918.1
• UniProt Accession #: P10827; P21205; Q8N6A1; Q96H73; A8K3B5
• Molecular Weight: 54,816 Da
• NCBI Official Full Name: thyroid hormone receptor alpha isoform 3
• NCBI Official Synonym Full Names: thyroid hormone receptor, alpha
• NCBI Official Symbol: THRA
• NCBI Official Synonym Symbols: AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
• NCBI Protein Information: thyroid hormone receptor alpha; EAR-7; c-erbA-alpha; ERBA-related 7; V-erbA-related protein 7; triiodothyronine receptor; nuclear receptor subfamily 1 group A member 1; thyroid normone nuclear receptor alpha variant 1; thyroid hormone receptor, alpha (ery
• UniProt Protein Name: Thyroid hormone receptor alpha
• UniProt Gene Name: THRA
• UniProt Synonym Gene Names: EAR7; ERBA1; NR1A1; THRA1; THRA2; EAR-7
• UniProt Entry Name: THA_HUMAN

NCBI Description

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TR-alpha: nuclear hormone receptor and transcription factor. High affinity receptor for triiodothyronine. Interacts with SRC-3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Four splice variant isoforms have been described.

Protein type: DNA-binding; Transcription factor; Nuclear receptor

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: protein domain specific binding; protein binding; zinc ion binding; chromatin DNA binding; sequence-specific DNA binding; TATA-binding protein binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of transcriptional preinitiation complex assembly; cytoplasmic sequestering of transcription factor; ossification; intracellular receptor-mediated signaling pathway; hormone-mediated signaling; positive regulation of female receptivity; regulation of myeloid cell apoptosis; regulation of heart contraction; regulation of lipid catabolic process; regulation of transcription from RNA polymerase II promoter; learning and/or memory; female courtship behavior; thyroid gland development; erythrocyte differentiation; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to cold; negative regulation of transcription, DNA-dependent; cartilage condensation

Disease: Hypothyroidism, Congenital, Nongoitrous, 6