Rabbit TRADD Polyclonal Antibody | anti-TRADD antibody

TRADD (Tumor Necrosis Factor Receptor 1 Associated Death Domain, Hs.89862, MGC11078) (PE)

Cat. Num. AAA6441565

• Gene Names: TRADD; Hs.89862
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: TRADD; Polyclonal Antibody; TRADD (Tumor Necrosis Factor Receptor 1 Associated Death Domain; Hs.89862; MGC11078) (PE); anti-TRADD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes TRADD. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-TRADD antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human TRADD.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Jurkat cell and Hela cell lysate using TRADD antibody.)

Product Categories/Family for anti-TRADD antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 13378137
• NCBI GeneID: 8717
• NCBI Accession #: NP_003780.1
• NCBI GenBank Nucleotide #: NM_003789.3
• UniProt Accession #: Q15628; Q52NZ1; B2RDS3
• Molecular Weight: 34,247 Da
• NCBI Official Full Name: tumor necrosis factor receptor type 1-associated DEATH domain protein
• NCBI Official Synonym Full Names: TNFRSF1A-associated via death domain
• NCBI Official Symbol: TRADD
• NCBI Official Synonym Symbols: Hs.89862
• NCBI Protein Information: tumor necrosis factor receptor type 1-associated DEATH domain protein; TNFR1-associated death domain protein; tumor necrosis factor receptor-1-associated protein; tumor necrosis factor receptor type 1 associated death domain protein
• UniProt Protein Name: Tumor necrosis factor receptor type 1-associated DEATH domain protein
• Protein Family: Tumor necrosis factor receptor type 1-associated DEATH domain protein
• UniProt Gene Name: TRADD
• UniProt Synonym Gene Names: TNFR1-associated DEATH domain protein
• UniProt Entry Name: TRADD_HUMAN

NCBI Description

The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

TRADD: Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. Heterodimer with TNFRSF1A/TNFR1. Interacts with DAB2IP, FADD, HIPK2, KRT14, KRT16, KRT17, KRT18, RIPK1, SQSTM1, TRAF1, TRAF2 and TRPC4AP. Found in all examined tissues.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: cytoskeleton; cytoplasm; nucleus; cytosol; receptor complex; lipid raft

Molecular Function: identical protein binding; signal transducer activity; protein binding; protein complex binding; tumor necrosis factor receptor binding; kinase binding; molecular adaptor activity

Biological Process: caspase activation; positive regulation of hair follicle development; positive regulation of I-kappaB kinase/NF-kappaB cascade; induction of apoptosis via death domain receptors; tumor necrosis factor-mediated signaling pathway; protein heterooligomerization; apoptosis; positive regulation of apoptosis; signal transduction; activation of NF-kappaB transcription factor