Rabbit HSF2 Polyclonal Antibody | anti-HSF2 antibody

HSF2 (Heat Shock Factor 2, HSF 2, Heat Shock Transcription Factor 2, HSTF 2) (HRP)

Cat. Num. AAA6421528

• Gene Names: HSF2; HSF 2; HSTF 2
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: HSF2; Polyclonal Antibody; HSF2 (Heat Shock Factor 2; HSF 2; Heat Shock Transcription Factor 2; HSTF 2) (HRP); anti-HSF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes HSF2. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-HSF2 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human HSF2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of HeLa cell line, using HSF2 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using HSF2 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-HSF2 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 207113147
• NCBI GeneID: 3298
• NCBI Accession #: NP_001129036.1
• NCBI GenBank Nucleotide #: NM_001135564.1
• UniProt Accession #: Q03933; Q0VAH9; Q2M1K4; Q9H445; B4DGJ4
• Molecular Weight: 60,348 Da
• NCBI Official Full Name: heat shock factor protein 2 isoform b
• NCBI Official Synonym Full Names: heat shock transcription factor 2
• NCBI Official Symbol: HSF2
• NCBI Official Synonym Symbols: HSF 2; HSTF 2
• NCBI Protein Information: heat shock factor protein 2
• UniProt Protein Name: Heat shock factor protein 2
• Protein Family: Heat shock factor protein
• UniProt Gene Name: HSF2
• UniProt Synonym Gene Names: HSTF2; HSF 2; HSTF 2
• UniProt Entry Name: HSF2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

HSF2: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Belongs to the HSF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6q22.31

Cellular Component: cytoplasm; nucleus

Molecular Function: protein homodimerization activity; transcription coactivator activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis