Rabbit TPM1 Polyclonal Antibody | anti-TPM1 antibody

TPM1 Antibody

Cat. Num. AAA9606950

• Gene Names: TPM1; CMH3; TMSA; CMD1Y; LVNC9; C15orf13; HEL-S-265; HTM-alpha
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: TPM1; Polyclonal Antibody; TPM1 Antibody; AA986836; AI854628; Alpha tropomyosin; alpha-TM; Alpha-tropomyosin; C15orf13; cardiomyopathy; hypertrophic 3; CMD1Y; CMH3; HTM alpha; HTM-alpha; OTTHUMP00000163688; sarcomeric tropomyosin kappa; TM2; Tmpa; TMSA; Tpm-1; TPM1_HUMAN; tropomyosin 1 (alpha); tropomyosin 1 (alpha) isoform 1; tropomyosin 1 (alpha) isoform 2; tropomyosin 1 (alpha) isoform 3; tropomyosin 1 (alpha) isoform 4; tropomyosin 1 (alpha) isoform 5; tropomyosin 1 (alpha) isoform 6; tropomyosin 1 (alpha) isoform 7; Tropomyosin 1; Tropomyosin alpha 1 chain; Tropomyosin alpha-1 chain; Tropomyosin; skeletal muscle alpha; Tropomyosin-1; anti-TPM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TPM1 antibody detects endogenous levels of total TPM1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 284

• Applicable Applications for anti-TPM1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human TPM1
• Subcellular Location: Cytoplasm > Cytoskeleton.
• Tissue Specificity: Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (92%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (92%), Xenopus (83%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9606950 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of Hepg2 whole cell lysates, using TPM1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-TPM1 antibody

Description: Tropomyosin-1 (TPM1) belongs to the high molecular weight members of tropomyosin family (1, 2). The protein exists in an alpha-helical coiled-coil conformation and binds multiple acting monomers in a tight manner to stabilize and regulate the actin filament (3). Tropomyosins fullfill functions in muscle and non-muscle cells. In muscle cells, tropomyosins associate with the troponin complex and play a central role in the calcium-dependent regulation of striated muscle contraction in vertebrates. In non-muscle cells, tropomyosins are implicated in the formation and stabilization of cytoskeletal actin filaments to ensure normal cellular processes (1, 2). Mutations of tropomysin-1 have been reported as a cause of dilated cardiac myopathies (4). Tropomyosin-1 also functions as a tumor suppressor, and many malignant tumors demonstrate downregulation of tropomyosin-1 expression (5-8). Tropomyosin-1 is phosphorylated at Ser283 through the Erk/DAPK pathway, which promotes stress fiber formation in response to oxidative stress (9-10).
Function: Binds to actin filaments in muscle and non-muscle cells (PubMed:23170982). Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction (PubMed:23170982). Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Subunit Structure: Homodimer (PubMed:23170982). Heterodimer of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain (By similarity). Interacts with HRG (via the HRR domain); the interaction contributes to the antiangiogenic properties of the histidine/proline-rich region (HRR) of HRG (By similarity). Interacts (via N-terminus) with LMOD2 (via N-terminus) and TMOD1 (via N-terminus) (PubMed:26873245).
Post-translational Modifications: Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells.
Similarity: The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity. Belongs to the tropomyosin family.

NCBI and Uniprot Product Information

• NCBI GI #: 27597085
• NCBI GeneID: 7168
• NCBI Accession #: NP_000357.3
• NCBI GenBank Nucleotide #: NM_000366.5
• UniProt Accession #: P09493; P09494; P10469; Q6DV89; Q6DV90; Q7Z6L8; Q86W64; Q96IK2; B7Z5T7; D9YZV2; D9YZV3; D9YZV8
• Molecular Weight: Observed: 33 kDa; Predicted: 33 kDa
• NCBI Official Full Name: tropomyosin alpha-1 chain isoform Tpm1.5cy
• NCBI Official Synonym Full Names: tropomyosin 1
• NCBI Official Symbol: TPM1
• NCBI Official Synonym Symbols: CMH3; TMSA; CMD1Y; LVNC9; C15orf13; HEL-S-265; HTM-alpha
• NCBI Protein Information: tropomyosin alpha-1 chain
• UniProt Protein Name: Tropomyosin alpha-1 chain
• Protein Family: Tropomyosin
• UniProt Gene Name: TPM1
• UniProt Synonym Gene Names: C15orf13; TMSA

NCBI Description

This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

Binds to actin filaments in muscle and non-muscle cells (PubMed:23170982). Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction (PubMed:23170982). Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.