Western Blot (WB)
(Western blot analysis of TPH2 Antibody (Center) in 293 cell line lysates (35ug/lane). TPH2 (arrow) was detected using the purified Pab.)
Rabbit anti-Human TPH2 Polyclonal Antibody | anti-TPH2 antibody
TPH2, ID (TPH2, NTPH, Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2) (AP)
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of TPH2 Antibody (Center) in 293 cell line lysates (35ug/lane). TPH2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of TPH2 Antibody (Center) in 293 cell line lysates (35ug/lane). TPH2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(TPH2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TPH2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(TPH2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TPH2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(TPH2 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(TPH2 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. [provided by RefSeq, Jul 2008]
Uniprot Description
TPH2: an enzyme that is rate-limiting in the biosynthesis of serotonin. It catalyzes the biopterin dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Its expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. Two alternatively spliced isoforms have been described. Isoform 2 appears to be expressed exclusively in the brain, and is expressed at significantly higher levels in the raphe nuclei than isoform 1.
Protein type: Amino Acid Metabolism - tryptophan; Oxidoreductase; EC 1.14.16.4
Chromosomal Location of Human Ortholog: 12q21.1
Cellular Component: neuron projection; cytosol
Molecular Function: amino acid binding; tryptophan 5-monooxygenase activity; iron ion binding
Biological Process: response to nutrient levels; circadian rhythm; indolalkylamine biosynthetic process; aromatic amino acid family metabolic process; response to estrogen stimulus; response to glucocorticoid stimulus; response to activity; response to calcium ion; serotonin biosynthetic process
Disease: Major Depressive Disorder; Attention Deficit-hyperactivity Disorder, Susceptibility To, 7
Research Articles on TPH2
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Product Notes
The TPH2 tph2 (Catalog #AAA6357626) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TPH2, ID (TPH2, NTPH, Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TPH2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TPH2 tph2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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