Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Topoisomerase II Polyclonal Antibody | anti-TOP2 antibody
Polyclonal Antibody to Topoisomerase II (TOP2)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GY DSDPVKAWKE AQQKVPEEEE NEENEESESE STSPAAESGP TFNYLLDMPL WYLTKEKKDE LCKQRDEKEQ ELNTLKKKTP SDLWKEDLAA FVEELEVVEA KEKQDEQVGL PGKGVKAKGK KAQISEVLPS PVGKRVIPQV TMEMRAEAEK KIRRKIKSEN VEGTPAEDGA EPGLRQRLEK RQKREPGTRA KKQTTLPFKP IKKAQKQNPW SDSESDMSSN ESNFDVPPRE KEPRIAATKA KFTADLDS
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue))
NCBI and Uniprot Product Information
NCBI Description
DNA topoisomerase, a nuclear enzyme that can alter the topologic state of DNA during transcription [RGD, Feb 2006]
Uniprot Description
TOP2A: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes (By similarity)
Protein type: EC 5.99.1.3; Isomerase
Chromosomal Location of Human Ortholog: 10q31
Cellular Component: centriole; condensed chromosome; DNA topoisomerase complex (ATP-hydrolyzing); intracellular ribonucleoprotein complex; nuclear chromosome; nucleolus; nucleoplasm; nucleus; protein complex
Molecular Function: ATP binding; chromatin binding; DNA binding; DNA binding, bending; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase activity; DNA-dependent ATPase activity; drug binding; enzyme binding; histone deacetylase binding; magnesium ion binding; protein C-terminus binding; protein heterodimerization activity; protein homodimerization activity; protein kinase C binding; RNA binding; sequence-specific DNA binding; ubiquitin binding
Biological Process: aging; apoptotic chromosome condensation; brain development; cellular response to DNA damage stimulus; cerebellar granule cell differentiation; cerebellar Purkinje cell differentiation; chromosome condensation; chromosome segregation; DNA ligation; DNA topological change; embryonic cleavage; hemopoietic progenitor cell differentiation; positive regulation of apoptosis; positive regulation of single stranded viral RNA replication via double stranded DNA intermediate; positive regulation of transcription from RNA polymerase II promoter; positive regulation of viral genome replication; regulation of circadian rhythm; resolution of meiotic recombination intermediates; response to drug; response to ionizing radiation; rhythmic process; sister chromatid segregation
Research Articles on TOP2
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Product Notes
The TOP2 top2a (Catalog #AAA2028540) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Topoisomerase II (TOP2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Topoisomerase II can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the TOP2 top2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GY DSDPVKAWKE AQQKVPEEEE NEENEESESE STSPAAESGP TFNYLLDMPL WYLTKEKKDE LCKQRDEKEQ ELNTLKKKTP SDLWKEDLAA FVEELEVVEA KEKQDEQVGL PGKGVKAKGK KAQISEVLPS PVGKRVIPQV TMEMRAEAEK KIRRKIKSEN VEGTPAEDGA EPGLRQRLEK RQKREPGTRA KKQTTLPFKP IKKAQKQNPW SDSESDMSSN ESNFDVPPRE KEPRIAATKA KFTADLDS. It is sometimes possible for the material contained within the vial of "Topoisomerase II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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