Rabbit anti-Human TOP2A / Topoisomerase II Alpha Polyclonal Antibody | anti-TOP2A antibody

Rabbit Polyclonal (IgG) to Human TOP2A / Topoisomerase II Alpha

Cat. Num. AAA247965

• Gene Names: TOP2A; TOP2; TP2A
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TOP2A / Topoisomerase II Alpha; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human TOP2A / Topoisomerase II Alpha; Anti-TOP2A / Topoisomerase II Alpha Antibody (aa1-50) IHC-plus; TOP2A; DNA topoisomerase II; 170 kD; TOP2; DNA gyrase; DNA topoisomerase 2-alpha; TP2A; Topoisomerase II Alpha; Human TOP2A; anti-TOP2A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TOP2A Antibody detects endogenous levels of total TOP2A protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1531

• Applicable Applications for anti-TOP2A antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:20000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human TOP2A (aa1-50).
• Immunogen: TOP2A / Topoisomerase II Alpha antibody was raised against synthetic peptide from human TOP2A (aa1-50).
• Antigen Modification: aa1-50
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-TOP2A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of A549 cells, using TOP2A Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from HT-29 cells, using TOP2A Antibody. The lane on the right is treated with the synthesized peptide.)

Product Categories/Family for anti-TOP2A antibody

Family: Topoisomerase

NCBI and Uniprot Product Information

• NCBI GI #: 19913406
• NCBI GeneID: 7153
• NCBI Accession #: NP_001058.2
• NCBI GenBank Nucleotide #: NM_001067.3
• UniProt Accession #: P11388; Q71UN1; Q71UQ5; Q9HB24; Q9HB25; Q9HB26; Q9UP44; Q9UQP9; B2RTS1
• Molecular Weight: 182,681 Da
• NCBI Official Full Name: DNA topoisomerase 2-alpha
• NCBI Official Synonym Full Names: topoisomerase (DNA) II alpha 170kDa
• NCBI Official Symbol: TOP2A
• NCBI Official Synonym Symbols: TOP2; TP2A
• NCBI Protein Information: DNA topoisomerase 2-alpha; DNA gyrase; DNA topoisomerase II, 170 kD; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase II, alpha isozyme
• UniProt Protein Name: DNA topoisomerase 2-alpha
• Protein Family: DNA topoisomerase
• UniProt Gene Name: TOP2A
• UniProt Synonym Gene Names: TOP2
• UniProt Entry Name: TOP2A_HUMAN

NCBI Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

Uniprot Description

TOP2A: a DNA topoisomerase. Controls of topological states of DNA by transient breakage and rejoining of DNA. Makes double-strand breaks. Four splice variant isoforms have been described.

Protein type: Isomerase; EC 5.99.1.3

Chromosomal Location of Human Ortholog: 17q21-q22

Cellular Component: centriole; nucleoplasm; nuclear chromosome; viral integration complex; protein complex; DNA topoisomerase complex (ATP-hydrolyzing); nucleolus; condensed chromosome; nucleus

Molecular Function: protein C-terminus binding; DNA-dependent ATPase activity; DNA topoisomerase (ATP-hydrolyzing) activity; ubiquitin binding; protein homodimerization activity; histone deacetylase binding; magnesium ion binding; drug binding; protein binding; enzyme binding; protein kinase C binding; DNA binding; protein heterodimerization activity; chromatin binding; DNA bending activity; ATP binding

Biological Process: DNA unwinding during replication; mitotic recombination; positive regulation of viral genome replication; DNA topological change; positive regulation of apoptosis; sister chromatid segregation; rhythmic process; regulation of circadian rhythm; apoptotic chromosome condensation; embryonic cleavage; chromosome segregation; DNA ligation; positive regulation of retroviral genome replication; resolution of meiotic joint molecules as recombinants; hemopoietic progenitor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; response to DNA damage stimulus