Rabbit Tollip Polyclonal Antibody | anti-TOLLIP antibody

Tollip Polyclonal Antibody

Cat. Num. AAA3008303

• Gene Names: TOLLIP; IL-1RAcPIP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Tollip; Polyclonal Antibody; Tollip Polyclonal Antibody; Toll-interacting protein; TOLLIP; anti-TOLLIP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TOLLIP polyclonal antibody detects endogenous levels of TOLLIP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 274

• Applicable Applications for anti-TOLLIP antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human Tollip.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Tollip polyclonal antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analysis of Tollip polyclonal antibody in paraffin-embedded Human Stomach tissue.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells, using Tollip polyclonal antibody)

Related Product Information for anti-TOLLIP antibody

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses. TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-kappaB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain.

NCBI and Uniprot Product Information

• NCBI GI #: 21361619
• NCBI GeneID: 54472
• NCBI Accession #: NP_061882.2
• NCBI GenBank Nucleotide #: NP_061882.2
• UniProt Accession #: Q9H0E2
• Molecular Weight: ~ 33kDa
• NCBI Official Full Name: toll-interacting protein isoform 1
• NCBI Official Synonym Full Names: toll interacting protein
• NCBI Official Symbol: TOLLIP
• NCBI Official Synonym Symbols: IL-1RAcPIP
• NCBI Protein Information: toll-interacting protein
• UniProt Protein Name: Toll-interacting protein
• Protein Family: Toll-interacting protein
• UniProt Gene Name: TOLLIP
• UniProt Entry Name: TOLIP_HUMAN

NCBI Description

This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

TOLLIP: Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2. Belongs to the tollip family.

Protein type: Inhibitor; Vesicle

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nuclear body; interleukin-1 receptor complex; perinuclear region of cytoplasm; cytoplasm; interleukin-18 receptor complex; cytosol

Molecular Function: protein binding; signal transducer activity; SUMO binding; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding; interleukin-1, Type I receptor binding; kinase binding

Biological Process: positive regulation of protein sumoylation; cell-cell signaling; epithelial cell differentiation; autophagy; innate immune response; signal transduction; inflammatory response; phosphorylation; leukocyte activation