Western Blot (WB)
(Western blot analysis of extracts of A-549 cells, using TLR4 antibody (MBS125491) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5min.)
Rabbit Toll-like Receptor 4 Polyclonal Antibody | anti-TLR4 antibody
Rabbit polyclonal to TLR4
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Applications
Western Blot, Immunofluorescence
Purity
Affinity purified
Synonyms
Toll-like Receptor 4; Polyclonal Antibody; Rabbit polyclonal to TLR4; TLR4 Rabbit pab Antibody; anti-TLR4 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
Liquid; PBS with 0.1% Sodium Azide, 50% Glycerol
Sequence Length
799
Applicable Applications for anti-TLR4 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 470-630 of human TLR4 (NP_612564.1).
Cross Reactivity
Human, Mouse
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of A-549 cells, using TLR4 antibody (MBS125491) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5min.)
Western Blot (WB)
(Western blot analysis of extracts of A-549 cells, using TLR4 antibody (MBS125491) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5min.)
Immunofluorescence (IF)
(Immunofluorescence analysis of RAW264.7 cells using TLR4 Polyclonal Antibody (MBS125491) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of RAW264.7 cells using TLR4 Polyclonal Antibody (MBS125491) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of THP-1 cells using TLR4 Polyclonal Antibody (MBS125491) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of THP-1 cells using TLR4 Polyclonal Antibody (MBS125491) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Related Product Information for anti-TLR4 antibody
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.
References
Akira, S. (2003) J Biol Chem 278, 38105-8.
Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 588-593.
Poltorak, A. et al. (1998) Science 282, 2085-2088.
Chow, J.C. et al. (1999) J. Biol. Chem. 274, 10689-10692.
Hoshino, K. et al. (1999) J. Immunol. 162, 3749-3752.
Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 588-593.
Poltorak, A. et al. (1998) Science 282, 2085-2088.
Chow, J.C. et al. (1999) J. Biol. Chem. 274, 10689-10692.
Hoshino, K. et al. (1999) J. Immunol. 162, 3749-3752.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed MW: 120 kDa
Calculated MW: 73 kDa/91 kDa/95 kDa
Calculated MW: 73 kDa/91 kDa/95 kDa
NCBI Official Full Name
toll-like receptor 4 isoform C
NCBI Official Synonym Full Names
toll-like receptor 4
NCBI Official Symbol
TLR4
NCBI Official Synonym Symbols
TOLL; CD284; TLR-4; ARMD10
NCBI Protein Information
toll-like receptor 4; hToll; homolog of Drosophila toll
UniProt Protein Name
Toll-like receptor 4
Protein Family
UniProt Gene Name
TLR4
UniProt Entry Name
TLR4_HUMAN
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Product Notes
The TLR4 tlr4 (Catalog #AAA125491) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Toll-like Receptor 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the TLR4 tlr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Toll-like Receptor 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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