Rabbit anti-Human TNIP2 Polyclonal Antibody | anti-TNIP2 antibody

TNIP2, ID (TNIP2, ABIN2, FLIP1, TNFAIP3-interacting protein 2, A20-binding inhibitor of NF-kappa-B activation 2, Fetal liver LKB1-interacting protein) (Biotin)

Cat. Num. AAA6357111

• Gene Names: TNIP2; KLIP; ABIN2; FLIP1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TNIP2; Polyclonal Antibody; ID (TNIP2; ABIN2; FLIP1; TNFAIP3-interacting protein 2; A20-binding inhibitor of NF-kappa-B activation 2; Fetal liver LKB1-interacting protein) (Biotin); Fetal liver LKB1-interacting protein; anti-TNIP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TNIP2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TNIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-194 amino acids from the Central region of human TNIP2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TNIP2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the TNIP2 antibody detected the TNIP2 protein (arrow).)

Related Product Information for anti-TNIP2 antibody

TNIP2 binds to the C-terminal zinc finger domain of A20 (TNFAIP3; MIM 191163) and is involved in activation of the ERK (see MAPK3; MIM 601795) MAP kinase pathway in various cell types (Van Huffel et al., 2001 [PubMed 11390377]; Papoutsopoulou et al., 2006 [PubMed 16633345]).

Product Categories/Family for anti-TNIP2 antibody

Antibodies; Binding Proteins

References

Raychaudhuri, S., et al. Nat. Genet. 41(12):1313-1318(2009). Handoyo, H., et al. Biochem. J. 424(1):109-118(2009). Verstrepen, L., et al. Biochem. Pharmacol. 78(2):105-114(2009). Huang, L., et al. Oncogene 27(47):6131-6140(2008). Liu, Y., et al. J. Radiat. Res. 48(1):13-20(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 239787094
• NCBI GeneID: 79155
• NCBI Accession #: NP_001154999.1; NP_077285.3
• NCBI GenBank Nucleotide #: NM_001161527.1
• UniProt Accession #: Q8NFZ5; Q7L5L2; Q9BQR6; Q9H682; B1AKS4; B3KTY8; D3DVQ9
• Molecular Weight: 36,682 Da
• NCBI Official Full Name: TNFAIP3-interacting protein 2 isoform 2
• NCBI Official Synonym Full Names: TNFAIP3 interacting protein 2
• NCBI Official Symbol: TNIP2
• NCBI Official Synonym Symbols: KLIP; ABIN2; FLIP1
• NCBI Protein Information: TNFAIP3-interacting protein 2; TNIP2; fetal liver LKB1-interacting protein; A20-binding inhibitor of NF-kappaB activation-2
• UniProt Protein Name: TNFAIP3-interacting protein 2
• Protein Family: TNFAIP3-interacting protein
• UniProt Gene Name: TNIP2
• UniProt Synonym Gene Names: ABIN-2
• UniProt Entry Name: TNIP2_HUMAN

NCBI Description

This gene encodes a protein which acts as an inhibitor of NFkappaB activation. The encoded protein is also involved in MAP/ERK signaling pathway in specific cell types. It may be involved in apoptosis of endothelial cells. Alternative splicing results in multiple transcript variants. A pseudogene related to this gene is located on the X chromosome.[provided by RefSeq, May 2014]

Uniprot Description

TNIP2: Inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector NEMO/IKBKG. Forms a ternary complex with NFKB1 and MAP3K8 but appears to function upstream of MAP3K8 in the TLR4 signaling pathway that regulates MAP3K8 activation. Involved in activation of the MEK/ERK signaling pathway during innate immune response; this function seems to be stimulus- and cell type specific. Required for stability of MAP3K8. Involved in regulation of apoptosis in endothelial cells; promotes TEK agonist-stimulated endothelial survival. May act as transcriptional coactivator when translocated to the nucleus. Enhances CHUK-mediated NF-kappa-B activation involving NF-kappa-B p50-p65 and p50-c-Rel complexes. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; polyubiquitin binding; protein kinase binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; protein stabilization; apoptosis; positive regulation of B cell activation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; toll-like receptor 3 signaling pathway; inflammatory response; positive regulation of macrophage activation; toll-like receptor 2 signaling pathway