Western Blot
(Western Blot analysis using beta Galactosidase antibodyWestern blotting using Fluorescein conj ugated beta Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of beta-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conj ugated beta Galactosidase antibody diluted to 1:10, 000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by Biorad. Other detection systems will yield similar results. )
Rabbit beta Galactosidase Polyclonal Antibody | anti-GLB1 antibody
beta Galactosidase antibody
Reactivity
To be determined by end-user
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Beta Galactosidase antibody was purified by delipidation and defibrination.
Synonyms
beta Galactosidase; Polyclonal Antibody; beta Galactosidase antibody; Polyclonal beta Galactosidase; Anti-beta Galactosidase; B-Gal; beta-Galactosidase; BGAL; B-Galactosidase; anti-GLB1 antibody
Host
Rabbit
Reactivity
To be determined by end-user
Clonality
Polyclonal
Purity/Purification
Beta Galactosidase antibody was purified by delipidation and defibrination.
Form/Format
Monospecific antisera, absorbed, delipidated and defibrinated, supplied in 0.02M NaPO4, 0.15M NaCl, pH 7.2, with 0.01% NaN3
Sequence Length
668
Applicable Applications for anti-GLB1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:65,000
WB: 1:500-1:2,000
WB: 1:500-1:2,000
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
Beta galactosidase antibody was raised in rabbit using beta galactosidase (E Coli) as the immunogen.
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Western Blot
(Western Blot analysis using beta Galactosidase antibodyWestern blotting using Fluorescein conj ugated beta Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of beta-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conj ugated beta Galactosidase antibody diluted to 1:10, 000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by Biorad. Other detection systems will yield similar results. )
Western Blot
(Western Blot analysis using beta Galactosidase antibodyWestern blotting using Fluorescein conj ugated beta Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of beta-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conj ugated beta Galactosidase antibody diluted to 1:10, 000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by Biorad. Other detection systems will yield similar results. )
Related Product Information for anti-GLB1 antibody
Rabbit polyclonal beta Galactosidase antibody
Product Categories/Family for anti-GLB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,992 Da
NCBI Official Full Name
beta-galactosidase
NCBI Official Symbol
GLB1
NCBI Protein Information
beta-galactosidase
UniProt Protein Name
Beta-galactosidase
Protein Family
UniProt Gene Name
GLB1
UniProt Synonym Gene Names
Lactase
UniProt Entry Name
BGAL_CANFA
Uniprot Description
Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.
Research Articles on GLB1
Similar Products
Product Notes
The GLB1 glb1 (Catalog #AAA535251) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta Galactosidase antibody reacts with To be determined by end-user and may cross-react with other species as described in the data sheet. AAA Biotech's beta Galactosidase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:65,000 WB: 1:500-1:2,000. Researchers should empirically determine the suitability of the GLB1 glb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta Galactosidase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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