Rabbit anti-Human HLA Class 2 Antigen DRB1 Polyclonal Antibody | anti-HLA-DR1B antibody

HLA Class 2 Antigen DRB1, NT (HLA Class II Histocompatibility Antigen DRB1-1 beta Chain, HLA-DR1B, HLA-DRB1, HLA-DRB1*, HLA-DRB, DR1, DR-1, DRB1, DRw10, FLJ75017, FLJ76359, MHC Class II Antigen DRB1*1, SS1) (Biotin)

Cat. Num. AAA6480966

• Gene Names: HLA-DRB1; SS1; DRB1; HLA-DRB; HLA-DR1B
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A Affinity Chromatography
• Synonyms: HLA Class 2 Antigen DRB1; Polyclonal Antibody; NT (HLA Class II Histocompatibility Antigen DRB1-1 beta Chain; HLA-DR1B; HLA-DRB1; HLA-DRB1*; HLA-DRB; DR1; DR-1; DRB1; DRw10; FLJ75017; FLJ76359; MHC Class II Antigen DRB1*1; SS1) (Biotin); Major Histocompatibility Complex; Class II; DR beta 1; anti-HLA-DR1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HLA Class 2 Antigen DRB1.
• Purity/Purification: Purified by Protein A Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-HLA-DR1B antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 30-58 in the N-terminal region of human HLA-DRB1, UniProt Accession #NP_002115; P04229; Q30167.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis using using MBS624953 in WiDr cell line lysates (35ug/lane). HLA-DRB1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western Blot analysis of lysates from NCI-H1299, Raji cell line (35ug/lane) (from left to right) (1:1000/lane) usingMBS624953. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of widr cells using MBS624953 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human brain tissue reacted withMBS624953, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Product Categories/Family for anti-HLA-DR1B antibody

Antibodies; HLA

References

1. Miyagawa, T., et.al., J. Hum. Genet. (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 155030211
• NCBI GeneID: 3123
• NCBI Accession #: NP_002115
• NCBI GenBank Nucleotide #: NM_002124.3
• UniProt Accession #: P04229; Q30167; P01911; P01912; P20039; Q29974; Q30134; Q5Y7A7; Q95IE3; Q9GIY3
• Molecular Weight: 25.2 kDa (219aa)
• NCBI Official Full Name: major histocompatibility complex, class II, DR beta 1
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DR beta 1
• NCBI Official Symbol: HLA-DRB1
• NCBI Official Synonym Symbols: SS1; DRB1; HLA-DRB; HLA-DR1B
• NCBI Protein Information: major histocompatibility complex, class II, DR beta 1
• UniProt Protein Name: HLA class II histocompatibility antigen, DRB1-1 beta chain
• UniProt Gene Name: HLA-DRB1
• UniProt Synonym Gene Names: DR-1; DR1
• UniProt Entry Name: 2B11_HUMAN

NCBI Description

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DRB1: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading. Genetic variation in HLA-DRB1 is a cause of susceptibility to sarcoidosis type 1 (SS1). Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; cell surface; membrane; integral to plasma membrane; late endosome membrane; lysosomal membrane; plasma membrane; trans-Golgi network membrane; external side of plasma membrane; MHC class II protein complex

Molecular Function: MHC class II receptor activity; peptide antigen binding

Biological Process: T-helper 1 type immune response; detection of bacterium; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; immunoglobulin production during immune response; T cell receptor signaling pathway; polysaccharide assembly with MHC class II protein complex; humoral immune response mediated by circulating immunoglobulin; negative regulation of T cell proliferation; inflammatory response to antigenic stimulus; peptide antigen assembly with MHC class II protein complex; regulation of interleukin-4 production; negative regulation of interferon-gamma production; T cell costimulation; immune response; protein tetramerization

Disease: Rheumatoid Arthritis; Sarcoidosis, Susceptibility To, 1; Multiple Sclerosis, Susceptibility To