Rabbit anti-Human TNFRSF25 Polyclonal Antibody | anti-TNFRSF25 antibody

TNFRSF25, ID (TNFRSF25, APO3, DDR3, DR3, TNFRSF12, WSL, WSL1, Tumor necrosis factor receptor superfamily member 25, Apo-3, Apoptosis-inducing receptor AIR, Apoptosis-mediating receptor DR3, Apoptosis-mediating receptor TRAMP, Death receptor 3, Lymphocyte-

Cat. Num. AAA6356992

• Gene Names: TNFRSF25; DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TNFRSF25; Polyclonal Antibody; ID (TNFRSF25; APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; Tumor necrosis factor receptor superfamily member 25; Apo-3; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; Death receptor 3; Lymphocyte-; Lymphocyte-associated receptor of death; Protein WSL; Protein WSL-1; anti-TNFRSF25 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TNFRSF25 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TNFRSF25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-242 amino acids from the Central region of human TNFRSF25.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TNFRSF25 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TNFRSF25 antibody detected the TNFRSF25 protein (arrow).)

Related Product Information for anti-TNFRSF25 antibody

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.

Product Categories/Family for anti-TNFRSF25 antibody

Antibodies; Apoptosis

References

Bayry, J. Nat Rev Rheumatol 6(2):67-68(2010). Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009). Andresdottir, M.B., et al. Clin Transplant 23(5):660-665(2009). Fang, L., et al. J. Exp. Med. 205(5):1037-1048(2008). Han, J.Y., et al. Mol. Cells 22(2):168-174(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4507569
• NCBI GeneID: 8718
• NCBI Accession #: NP_003781.1; NP_001034753.1
• NCBI GenBank Nucleotide #: NM_003790.2
• UniProt Accession #: Q93038; O00275; O00276; O00277; O00278; O00279; O00280; O14865; O14866; B1ALX2; B1ALX3; B7ZLL7
• Molecular Weight: 29,111 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 25 isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 25
• NCBI Official Symbol: TNFRSF25
• NCBI Official Synonym Symbols: DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 25; protein WSL-1; death receptor beta; apoptosis inducing receptor; apoptosis-inducing receptor AIR; apoptosis-mediating receptor DR3; apoptosis-mediating receptor TRAMP; death domain receptor 3 soluble f
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 25
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF25
• UniProt Synonym Gene Names: APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; LARD
• UniProt Entry Name: TNR25_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF25: Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4. Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; cytosol

Molecular Function: tumor necrosis factor receptor activity; receptor activity

Biological Process: regulation of apoptosis; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; apoptosis; signal transduction