Rabbit TNFalpha-IP8 Polyclonal Antibody | anti-TNFalpha-IP8 antibody

TNFalpha-IP8 Polyclonal Antibody

Cat. Num. AAA3006271

• Gene Names: TNFAIP8; NDED; GG2-1; SCCS2; SCC-S2; MDC-3.13
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TNFalpha-IP8; Polyclonal Antibody; TNFalpha-IP8 Polyclonal Antibody; Tumor necrosis factor alpha-induced protein 8; TNF alpha-induced protein 8; Head and neck tumor and metastasis-related protein; MDC-3.13; MDC3.13; NF-kappa-B-inducible DED-containing protein; NDED; SCC-S2; SCC S2; SCCS2; TNF-induced protein GG2-1; TNFAIP8; TNFalpha IP8; TNFalphaIP8; TNFalpha-IP 8; anti-TNFalpha-IP8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TNFalpha-IP8 polyclonal antibody detects endogenous levels of TNFalpha-IP8 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 188

• Applicable Applications for anti-TNFalpha-IP8 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 71-116 of Human TNFalpha-IP83.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TNFalpha-IP8 polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:Raw264. 7 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-TNFalpha-IP8 antibody

TNFalpha-IP 8 (tumor necrosis factor, alpha-induced protein 8), also known as NFkappaB-inducible DED-containing protein (NDED), SCC-S2 or TNF-induced protein GG2-1, is a 198 amino acid cytoplasmic protein induced by NFkappaB and TNF. The induction of TNFalpha-IP 8 by TNF is dependent on the activation of NFkappaB. TNFalpha-IP 8 negatively mediates apoptosis and may also play a role in tumor progression. TNFalpha-IP 8 specifically inhibits caspase-8 activity, which results in the inhibition of BID cleavage and caspase-3 activation during TNF-mediated apoptosis. TNFalpha-IP 8 is expressed at high levels in thymus, bone marrow, lymph node, spleen, thyroid, placenta and various tumor tissues, as well as fetal lung, liver and kidney. TNFalpha-IP 8 is present as three isoforms produced by alternative splicing.

NCBI and Uniprot Product Information

• NCBI GI #: 117676370
• NCBI GeneID: 25816
• NCBI Accession #: NP_001071122.1
• NCBI GenBank Nucleotide #: NP_001071122.1
• UniProt Accession #: O95379
• Molecular Weight: ~ 23kDa
• NCBI Official Full Name: tumor necrosis factor alpha-induced protein 8 isoform b
• NCBI Official Synonym Full Names: TNF alpha induced protein 8
• NCBI Official Symbol: TNFAIP8
• NCBI Official Synonym Symbols: NDED; GG2-1; SCCS2; SCC-S2; MDC-3.13
• NCBI Protein Information: tumor necrosis factor alpha-induced protein 8
• UniProt Protein Name: Tumor necrosis factor alpha-induced protein 8
• UniProt Gene Name: TNFAIP8
• UniProt Synonym Gene Names: TNF alpha-induced protein 8; NDED
• UniProt Entry Name: TFIP8_HUMAN

Uniprot Description

TNFAIP8: Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. Belongs to the TNFAIP8 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 5q23.1

Cellular Component: cytoplasm

Molecular Function: caspase inhibitor activity

Biological Process: defense response to Gram-positive bacterium; interleukin-1 beta production; negative regulation of caspase activity; negative regulation of apoptosis