Western Blot (WB)
(TNFAIP8 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TNFAIP8 antibody detected the TNFAIP8 protein (arrow).)
Rabbit anti-Human TNFAIP8 Polyclonal Antibody | anti-TNFAIP8 antibody
TNFAIP8, NT (TNFAIP8, Tumor necrosis factor alpha-induced protein 8, Head and neck tumor and metastasis-related protein, MDC-3.13, NF-kappa-B-inducible DED-containing protein, SCC-S2, TNF-induced protein GG2-1)
Gene Names
TNFAIP8; GG2-1; SCCS2; SCC-S2; MDC-3.13
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
TNFAIP8; Polyclonal Antibody; NT (TNFAIP8; Tumor necrosis factor alpha-induced protein 8; Head and neck tumor and metastasis-related protein; MDC-3.13; NF-kappa-B-inducible DED-containing protein; SCC-S2; TNF-induced protein GG2-1); Anti -TNFAIP8; anti-TNFAIP8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-TNFAIP8 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Immunogen
TNFAIP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the N-terminal region of human TNFAIP8.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(TNFAIP8 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TNFAIP8 antibody detected the TNFAIP8 protein (arrow).)
Western Blot (WB)
(TNFAIP8 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TNFAIP8 antibody detected the TNFAIP8 protein (arrow).)
Flow Cytometry (FC/FACS)
(TNFAIP8 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(TNFAIP8 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-TNFAIP8 antibody
Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation.
Product Categories/Family for anti-TNFAIP8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,003 Da
NCBI Official Full Name
tumor necrosis factor alpha-induced protein 8 isoform b
NCBI Official Synonym Full Names
tumor necrosis factor, alpha-induced protein 8
NCBI Official Symbol
TNFAIP8
NCBI Official Synonym Symbols
GG2-1; SCCS2; SCC-S2; MDC-3.13
NCBI Protein Information
tumor necrosis factor alpha-induced protein 8; NDED; TNF-induced protein GG2-1; TNF alpha-induced protein 8; NF-kappa-B-inducible DED-containing protein; head and neck tumor and metastasis-related protein
UniProt Protein Name
Tumor necrosis factor alpha-induced protein 8
Protein Family
UniProt Gene Name
TNFAIP8
UniProt Synonym Gene Names
TNF alpha-induced protein 8; NDED
UniProt Entry Name
TFIP8_HUMAN
Uniprot Description
TNFAIP8: Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. Belongs to the TNFAIP8 family. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 5q23.1
Cellular Component: cytoplasm
Molecular Function: caspase inhibitor activity
Biological Process: defense response to Gram-positive bacterium; interleukin-1 beta production; negative regulation of caspase activity; negative regulation of apoptosis
Research Articles on TNFAIP8
Similar Products
TNFAIP8 Antibody
Host: Rabbit
Reactivity: Human
Apps: Flow Cytometry, Functional Assay, Western Blot
TNFAIP8 Antibody
Host: Rabbit
Reactivity: Human
Apps: Flow Cytometry, Functional Assay, Western Blot
Product Notes
The TNFAIP8 tnfaip8 (Catalog #AAA6012165) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TNFAIP8, NT (TNFAIP8, Tumor necrosis factor alpha-induced protein 8, Head and neck tumor and metastasis-related protein, MDC-3.13, NF-kappa-B-inducible DED-containing protein, SCC-S2, TNF-induced protein GG2-1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TNFAIP8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the TNFAIP8 tnfaip8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNFAIP8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
