Rabbit anti-Human, Mouse TNFAIP1 Polyclonal Antibody | anti-TNFAIP1 antibody

TNFAIP1, CT (TNFAIP1, BACURD2, EDP1, BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2, BTB/POZ domain-containing protein TNFAIP1, Protein B12, Tumor necrosis factor, alpha-induced protein 1, endothelial) (PE)

Cat. Num. AAA6356844

• Gene Names: TNFAIP1; B12; B61; EDP1; BTBD34; hBACURD2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TNFAIP1; Polyclonal Antibody; CT (TNFAIP1; BACURD2; EDP1; BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2; BTB/POZ domain-containing protein TNFAIP1; Protein B12; Tumor necrosis factor; alpha-induced protein 1; endothelial) (PE); endothelial; anti-TNFAIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TNFAIP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TNFAIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-324 amino acids from the C-terminal region of human TNFAIP1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TNFAIP1 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the TNFAIP1 antibody detected the TNFAIP1 protein (arrow).)

Related Product Information for anti-TNFAIP1 antibody

This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner.

Product Categories/Family for anti-TNFAIP1 antibody

Antibodies; Growth Factors; Cytokines

References

Grinchuk, O.V., et al. BMC Genomics 11 SUPPL 1, S9 (2010): Kim, D.M., et al. Int. J. Cancer 125(11):2520-2527(2009). Castro-Giner, F., et al. Eur. Respir. J. 33(5):1003-1009(2009). Yang, L.P., et al. Yi Chuan 28(8):918-922(2006). Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006). Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006). Suzuki, H., et al. Genome Res. 11(10):1758-1765(2001). Pandey, A., et al. Science 268(5210):567-569(1995) Wolf, F.W., et al. J. Biol. Chem. 267(2):1317-1326(1992) Holzman, L.B., et al. Mol. Cell. Biol. 10(11):5830-5838(1990) Dixit, V.M., et al. J. Biol. Chem. 265(5):2973-2978(1990).

NCBI and Uniprot Product Information

• NCBI GI #: 10863937
• NCBI GeneID: 7126
• NCBI Accession #: NP_066960.1
• NCBI GenBank Nucleotide #: NM_021137.4
• UniProt Accession #: Q13829; Q5TZQ1
• Molecular Weight: 36,204 Da
• NCBI Official Full Name: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
• NCBI Official Synonym Full Names: tumor necrosis factor, alpha-induced protein 1 (endothelial)
• NCBI Official Symbol: TNFAIP1
• NCBI Official Synonym Symbols: B12; B61; EDP1; BTBD34; hBACURD2
• NCBI Protein Information: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2; BTB/POZ domain-containing protein TNFAIP1
• UniProt Protein Name: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
• Protein Family: BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein
• UniProt Gene Name: TNFAIP1
• UniProt Synonym Gene Names: BACURD2; EDP1; hBACURD2
• UniProt Entry Name: BACD2_HUMAN

NCBI Description

This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFAIP1: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity. Belongs to the BACURD family.

Protein type: DNA replication; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 17q22-q23

Cellular Component: cytoplasm; nucleolus; endosome

Molecular Function: protein domain specific binding; protein binding; ubiquitin-protein ligase activity; GTP-Rho binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; cell migration; embryonic development; negative regulation of Rho protein signal transduction; apoptosis; stress fiber formation; protein ubiquitination; immune response; DNA replication; protein homooligomerization; positive regulation of DNA replication