Western Blot (WB)
(Anti-TNF Receptor II antibody, MBS176278, Western blottingLane 1: Rat Spleen Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: HEPA Cell Lysate )
Rabbit anti-Mouse, Rat TNF Receptor II Polyclonal Antibody | anti-TNFRSF1B antibody
Anti-TNF Receptor II antibody
Gene Names
Tnfrsf1b; p75; TNFBR; Tnfr2; CD120b; TNF-R2; TNFR80; TNFRII; Tnfr-1; TNF-R75; TNF-R-II; TNF-alphaR2; TNFalpha-R2
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
TNF Receptor II; Polyclonal Antibody; Anti-TNF Receptor II antibody; Tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily; member 1B; CD 120b antibody; CD120b antibody; CD120b antigen antibody; Etanercept antibody; p75 antibody; p75TNFR antibody; p80 TNF alpha receptor antibody; p80 TNF-alpha receptor antibody; TBP-2 antibody; TBP2 antibody; TBPII antibody; TNF R II antibody; TNF R2 antibody; TNF R75 antibody; TNF-R2 antibody; TNF-RII antibody; TNFBR antibody; TNFR 2 antibody; TNFR-II antibody; TNFR2 antibody; TNFR75 antibody; TNFR80 antibody; TNFRII antibody; TNFRSF 1B antibody; TNFRSF1B antibody; TNR1B antibody; TNR1B_HUMAN antibody; Tumor necrosis factor beta receptor antibody; Tumor necrosis factor binding protein 2 antibody; Tumor necrosis factor receptor 2 antibody; Tumor necrosis factor receptor superfamily member 1B precursor antibody; Tumor necrosis factor receptor type II antibody; Tumor necrosis factor-binding protein 2 antibody; anti-TNFRSF1B antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
474
Applicable Applications for anti-TNFRSF1B antibody
Western Blot (WB)
Application Notes
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Ms, Rat
WB: The detection limit for TNFRSF1B is approximately 1ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Concentration: 0.1-0.5ug/ml
Tested Species: Ms, Rat
WB: The detection limit for TNFRSF1B is approximately 1ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse TNF Receptor II (38-55aa YECQISQEYYDRKAQMCC), different from the related rat sequence by three amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-TNF Receptor II antibody, MBS176278, Western blottingLane 1: Rat Spleen Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: HEPA Cell Lysate )
Western Blot (WB)
(Anti-TNF Receptor II antibody, MBS176278, Western blottingLane 1: Rat Spleen Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: HEPA Cell Lysate )
Related Product Information for anti-TNFRSF1B antibody
Description: Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 1B(TNFRSF1B) detection. Tested with WB in Mouse, Rat.
Background: TNFRSF1B(Tumor Necrosis Factor Receptor Subfamily Member 1B), also known as TNFR2 or TNFBR, is a protein that in humans is encoded by the TNFRSF1B gene. The protein encoded by this gene is a member of the Tumor necrosis factor receptor superfamily, which also contains TNFRSF1A. By linkage analysis, Santee and Owen Schaub(1996) confirmed the mapping of the TNFR2 gene to 1p36.3-p36.2. TNFBR(TNFR75) is the larger of the 2 TNF receptors. It is present on many cell types, especially those of myeloid origin, and is strongly expressed on stimulated T and B lymphocytes. Beltinger et al.(1996) noted that TNFR2 is the main TNF receptor found on circulating T cells and is the major mediator of autoregulatory apoptosis in CD8+ cells. TNFR2 may act with TNFR1 to kill nonlymphoid cells.
Background: TNFRSF1B(Tumor Necrosis Factor Receptor Subfamily Member 1B), also known as TNFR2 or TNFBR, is a protein that in humans is encoded by the TNFRSF1B gene. The protein encoded by this gene is a member of the Tumor necrosis factor receptor superfamily, which also contains TNFRSF1A. By linkage analysis, Santee and Owen Schaub(1996) confirmed the mapping of the TNFR2 gene to 1p36.3-p36.2. TNFBR(TNFR75) is the larger of the 2 TNF receptors. It is present on many cell types, especially those of myeloid origin, and is strongly expressed on stimulated T and B lymphocytes. Beltinger et al.(1996) noted that TNFR2 is the main TNF receptor found on circulating T cells and is the major mediator of autoregulatory apoptosis in CD8+ cells. TNFR2 may act with TNFR1 to kill nonlymphoid cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
50,320 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 1B
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 1b
NCBI Official Symbol
Tnfrsf1b
NCBI Official Synonym Symbols
p75; TNFBR; Tnfr2; CD120b; TNF-R2; TNFR80; TNFRII; Tnfr-1; TNF-R75; TNF-R-II; TNF-alphaR2; TNFalpha-R2
NCBI Protein Information
tumor necrosis factor receptor superfamily member 1B; TNF-RII; TNFR-II; p75 TNFR; p80 TNF-alpha receptor; TNF receptor beta chain; tumor necrosis factor receptor 2; tumor necrosis factor receptor type II
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 1B
Protein Family
UniProt Gene Name
Tnfrsf1b
UniProt Synonym Gene Names
Tnfr-2; Tnfr2; TNF-R2; TNF-RII; TNFR-II
UniProt Entry Name
TNR1B_MOUSE
Uniprot Description
TNF-R2: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. Binds to TRAF2. Interacts with BMX. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, cytokine
Cellular Component: cell soma; membrane; axon; perinuclear region of cytoplasm; integral to membrane; intracellular; nucleus; lipid raft
Molecular Function: protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding
Biological Process: cell proliferation; cell surface receptor linked signal transduction; DNA damage response, signal transduction resulting in induction of apoptosis; tumor necrosis factor-mediated signaling pathway; negative regulation of inflammatory response; programmed cell death; immune response; inflammatory response; RNA destabilization; positive regulation of membrane protein ectodomain proteolysis
Research Articles on TNFRSF1B
Similar Products
Product Notes
The TNFRSF1B tnfrsf1b (Catalog #AAA176278) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TNF Receptor II antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TNF Receptor II can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Ms, Rat WB: The detection limit for TNFRSF1B is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the TNFRSF1B tnfrsf1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF Receptor II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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