Rabbit TNF-R1 Polyclonal Antibody | anti-TNFRSF1A antibody

TNF-R1 Antibody: ATTO 594

Cat. Num. AAA805315

• Gene Names: TNFRSF1A; FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2
• Reactivity: Human, Mouse, Rat, Monkey, Bovine, Canine, Rabbit
• Applications: Western Blot, Immunoprecipitation
• Synonyms: TNF-R1; Polyclonal Antibody; TNF-R1 Antibody: ATTO 594; Tumor necrosis factor receptor 1; TNFR-1; TNFRSF1A; TNFAR; TNFR1; anti-TNFRSF1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey, Bovine, Canine, Rabbit
• Clonality: Polyclonal
• Form/Format: Peptide Affinity Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 455

• Applicable Applications for anti-TNFRSF1A antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: 1:1000 (WB)
• Conjugate: ATTO 594
• Storage Buffer: PBS pH 7.4; 50% glycerol, 0.09% azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: dermis.)

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Punctate nuclear staining, dotty staining in cytoplasm.)

Related Product Information for anti-TNFRSF1A antibody

Background Info: Identifies a band ~55kD on WB

Scientific Background: The Tumor Necrosis Factor Receptor (TNFR) also known as Cluster of differentiation (CD120) is a protein that belongs to the (TNF)/ (TNFR) superfamily. TNF interacts with two distinct receptors TNFR1 and TNFR2. These receptors share no homology on their cytoplasmic sequences(1,3).TNFR1 also known as p55/p60 is a high affinity receptor for TNF-alpha. The TNFR1 has an extracellular domain with variable numbers of cysteine-rich repeats. The functional properties of TNFR1 are targets in new therapies for osteoporosis, chronic inflammatory and autoimmune diseases (1, 2). The TNF-alpha/TNFR1 receptor complex is responsible for the recruitment and the subsequent activation of the caspase (aspartate-specific cysteine proteases) that regulate apoptosis.

Product Categories/Family for anti-TNFRSF1A antibody

Cell Signaling

References

1. Kontermann RE., et al. (2008) J Immunother 31(3):225-34. 2. Hehlgans T. and Pfeffer K.(2005) Immunology 115(1):1-20. 3. 3.Al-Lamki S.,et al. (2005) The Faseb Journal 19:1638-1645.

NCBI and Uniprot Product Information

• NCBI GI #: 4507575
• NCBI GeneID: 7132
• NCBI Accession #: NP_001056.1
• NCBI GenBank Nucleotide #: NM_001065.3
• UniProt Accession #: P19438; Q9UCA4; A8K4X3; B2RDE4; B3KPQ1; B4DQB7; B4E309; B5M0B5; D3DUR1
• Molecular Weight: 24,194 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 1A
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 1A
• NCBI Official Symbol: TNFRSF1A
• NCBI Official Synonym Symbols: FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1A; TNF-R1; TNF-RI; TNFR-I; tumor necrosis factor-alpha receptor; tumor necrosis factor receptor type 1; tumor necrosis factor binding protein 1; tumor necrosis factor receptor 1A isoform beta
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1A
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF1A
• UniProt Synonym Gene Names: TNFAR; TNFR1; TNF-R1; TNF-RI; TNFR-I; TBPI
• UniProt Entry Name: TNR1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease. [provided by RefSeq, Jul 2008]

Uniprot Description

TNF-R1: Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD. Various TRADD-interacting proteins such as TRAFS, RIPK1 and possibly FADD, are recruited to the complex by their association with TRADD. This complex activates at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4, BRE, FEM1B, GRB2, SQSTM1 and TRPC4AP. Interacts with HCV core protein. Interacts with human cytomegalovirus/HHV-5 protein UL138. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: Golgi membrane; extracellular space; cell surface; mitochondrion; axon; integral to plasma membrane; plasma membrane; extracellular region; synapse; cytosol; nucleus; receptor complex; lipid raft

Molecular Function: protein binding; tumor necrosis factor receptor activity; protease binding; protein complex binding; tumor necrosis factor binding

Biological Process: response to alkaloid; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein import into nucleus, translocation; viral reproduction; protein heterooligomerization; cytokine and chemokine mediated signaling pathway; response to lipopolysaccharide; positive regulation of tumor necrosis factor production; response to amino acid stimulus; prostaglandin metabolic process; positive regulation of angiogenesis; positive regulation of tyrosine phosphorylation of Stat1 protein; response to ethanol; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; tumor necrosis factor-mediated signaling pathway; negative regulation of inflammatory response; positive regulation of neuron apoptosis; defense response to bacterium; response to hypoxia; response to wounding; negative regulation of interleukin-6 production; tetrapyrrole metabolic process; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of apoptosis; positive regulation of inflammatory response

Disease: Periodic Fever, Familial, Autosomal Dominant; Multiple Sclerosis, Susceptibility To, 5