Rabbit TNF ALPHA Polyclonal Antibody | anti-TNF ALPHA antibody

RABBIT ANTI RAT TNF ALPHA

Cat. Num. AAA221295

• Gene Names: Tnf; Tnfa; RATTNF; TNF-alpha
• Applications: ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: TNF ALPHA; Polyclonal Antibody; RABBIT ANTI RAT TNF ALPHA; anti-TNF ALPHA antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0 mg/ml after reconstitution. (varies by lot)
• Sequence Length: 235

• Applicable Applications for anti-TNF ALPHA antibody: ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin, Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0 ug/ml;; Functional Assays: Minimum Dilution: 0.07; Maximum Dilution: 0.1 ug/ml;; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2 ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Rat
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Related Product Information for anti-TNF ALPHA antibody

Rabbit anti Rat TNF alpha antibody recognizes rat tumor necrosis factor alpha (TNFalpha), also known as Cachectin or Tumor necrosis factor ligand superfamily member 2. Rat TNFalpha is a 235 amino acid ~17kDa single pass type II O-linked transmembrane glycoprotein, it also exists as a secreted cytokine through cleavage between residues 79 and 80. TNFalpha is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells.

NCBI and Uniprot Product Information

• NCBI GI #: 6981662
• NCBI GeneID: 24835
• NCBI Accession #: NP_036807.1
• NCBI GenBank Nucleotide #: NM_012675.3
• UniProt Accession #: P16599; Q6EE11; Q9JI26; Q9JI27
• Molecular Weight: 25,806 Da
• NCBI Official Full Name: tumor necrosis factor
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: Tnf
• NCBI Official Synonym Symbols: Tnfa; RATTNF; TNF-alpha
• NCBI Protein Information: tumor necrosis factor; cachectin; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2
• UniProt Protein Name: Tumor necrosis factor
• UniProt Gene Name: Tnf
• UniProt Synonym Gene Names: Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_RAT

NCBI Description

acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]

Uniprot Description

TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.

Protein type: Motility/polarity/chemotaxis; Cytokine; Apoptosis; Membrane protein, integral

Cellular Component: extracellular space; recycling endosome; cell surface; integral to plasma membrane; integral to membrane; plasma membrane; intracellular; lipid raft; phagocytic cup; external side of plasma membrane

Molecular Function: identical protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; positive regulation of NFAT protein import into nucleus; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; positive regulation of translational initiation by iron; positive regulation of membrane protein ectodomain proteolysis; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; tumor necrosis factor-mediated signaling pathway; cellular extravasation; positive regulation of phagocytosis; negative regulation of interleukin-6 production; JNK cascade; negative regulation of osteoblast differentiation; positive regulation of action potential; embryonic gut development; negative regulation of protein complex disassembly; positive regulation of cytokine production; response to drug; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; response to virus; positive regulation of interleukin-6 production; glucose metabolic process; negative regulation of cytokine secretion during immune response; positive regulation of protein transport; DNA damage response, signal transduction resulting in induction of apoptosis; response to mechanical stimulus; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; skeletal muscle contraction; sequestering of triacylglycerol; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-18 production; signal transduction; chronic inflammatory response to antigenic stimulus; positive regulation of hair follicle development; negative regulation of cell proliferation; positive regulation of neuron apoptosis; protein kinase B signaling cascade; positive regulation of chronic inflammatory response to antigenic stimulus; inflammatory response; regulation of protein amino acid phosphorylation; transformed cell apoptosis; positive regulation of peptidyl-serine phosphorylation; humoral immune response; regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of interferon-gamma production; positive regulation of programmed cell death; positive regulation of protein complex assembly; negative regulation of viral genome replication; response to hypoxia; regulation of insulin secretion; positive regulation of JNK activity; positive regulation of osteoclast differentiation; multicellular organismal development; response to glucocorticoid stimulus; positive regulation of NF-kappaB import into nucleus; response to lipopolysaccharide; osteoclast differentiation; regulation of immunoglobulin secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; positive regulation of chemokine production; cell activation; detection of mechanical stimulus involved in sensory perception of pain; organ morphogenesis; defense response to Gram-positive bacterium; induction of apoptosis via death domain receptors; positive regulation of transcription factor activity; response to activity; negative regulation of L-glutamate transport; negative regulation of transcription, DNA-dependent; leukocyte migration; apoptosis; positive regulation of smooth muscle cell proliferation; defense response; positive regulation of synaptic transmission; response to radiation; regulation of osteoclast differentiation; regulation of protein secretion; negative regulation of lipid catabolic process; lipopolysaccharide-mediated signaling pathway; regulation of I-kappaB kinase/NF-kappaB cascade; caspase activation; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of protein complex disassembly; calcium-mediated signaling; MAPKKK cascade; negative regulation of glucose import; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; positive regulation of protein kinase activity; activation of MAPKKK activity; positive regulation of fever; receptor biosynthetic process; positive regulation of protein amino acid phosphorylation; leukocyte tethering or rolling; negative regulation of myoblast differentiation; positive regulation of inflammatory response; positive regulation of cytokine secretion