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Western Blot (WB) ((1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat TMPRSS4 Polyclonal Antibody | anti-TMPRSS4 antibody

Goat anti-TMPRSS4 (aa245-57) Antibody

Gene Names
TMPRSS4; CAPH2; MT-SP2; TMPRSS3
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
TMPRSS4; Polyclonal Antibody; Goat anti-TMPRSS4 (aa245-57) Antibody; Goat Anti-TMPRSS4 Antibody; TMPRSS3; transmembrane protease; serine 4; MT-SP2; membrane-type serine protease 2; transmembrane serine protease 3; type II membrane serine protease; TMPRSS3 antibody; serine 4 antibody; MT-SP2 antibody; membrane-type serine protease 2 antibody; transmembrane serine protease 3 antibody; type II membrane serine protease antibody; TMPRSS4 (aa245-57); anti-TMPRSS4 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
HCFRKHTDVFNWK
Sequence Length
437
Applicable Applications for anti-TMPRSS4 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 1000.
Western Blot: approx. 48kDa band observed in lysates of cell lines HEK293 and A549 (calculated MW of 48.2kDa according to NP_063947.1). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence HCFRKHTDVFNWK, from the internal region of the protein sequence according to NP_063947.1; NP_001077416.1.
Epitope
Internal region
Note
This antibody is expected to recognise both reported isoforms (NP_063947.1 and NP_001077416.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,786 Da
NCBI Official Full Name
transmembrane protease serine 4 isoform 1
NCBI Official Synonym Full Names
transmembrane protease, serine 4
NCBI Official Symbol
TMPRSS4
NCBI Official Synonym Symbols
CAPH2; MT-SP2; TMPRSS3
NCBI Protein Information
transmembrane protease serine 4
UniProt Protein Name
Transmembrane protease serine 4
UniProt Gene Name
TMPRSS4
UniProt Synonym Gene Names
TMPRSS3; CAPH2; MT-SP2
UniProt Entry Name
TMPS4_HUMAN

NCBI Description

This gene encodes a member of the serine protease family. Serine proteases are known to be involved in a variety of biological processes, whose malfunction often leads to human diseases and disorders. This gene was identified as a gene overexpressed in pancreatic carcinoma. The encoded protein is membrane bound with a N-terminal anchor sequence and a glycosylated extracellular region containing the serine protease domain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TMPRSS4: Probable protease. Seems to be capable of activating ENaC. Belongs to the peptidase S1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.4.21.-; Protease

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: integral to membrane

Molecular Function: protein binding; scavenger receptor activity; serine-type endopeptidase activity

Biological Process: proteolysis; receptor-mediated endocytosis

Research Articles on TMPRSS4

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Product Notes

The TMPRSS4 tmprss4 (Catalog #AAA422046) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TMPRSS4 (aa245-57) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's TMPRSS4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 1000. Western Blot: approx. 48kDa band observed in lysates of cell lines HEK293 and A549 (calculated MW of 48.2kDa according to NP_063947.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the TMPRSS4 tmprss4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HCFRKHTDVF NWK. It is sometimes possible for the material contained within the vial of "TMPRSS4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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