Human Stanniocalcin 2 (STC2) ELISA Kit | STC2 elisa kit

Human Stanniocalcin 2 (STC2) ELISA Kit

Cat. Num. AAA2705962

• Gene Names: STC2; STC-2; STCRP
• Reactivity: Human
• Synonyms: Stanniocalcin 2 (STC2); Human Stanniocalcin 2 (STC2) ELISA Kit; STCRP; Stanniocalcin-related protein; STC2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Stanniocalcin 2 (STC2).; No significant cross-reactivity or interference between Stanniocalcin 2 (STC2) and analogues was observed.
• Sequence Length: 302

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 31.2-2,000pg/mL
• Sensitivity: < 13.6pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stanniocalcin 2 (STC2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stanniocalcin 2 (STC2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STC2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stanniocalcin 2 (STC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stanniocalcin 2 (STC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stanniocalcin 2 (STC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stanniocalcin 2 (STC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15530321
• NCBI GeneID: 8614
• UniProt Accession #: O76061
• Molecular Weight: 33,249 Da
• NCBI Official Full Name: Stanniocalcin 2
• NCBI Official Synonym Full Names: stanniocalcin 2
• NCBI Official Symbol: STC2
• NCBI Official Synonym Symbols: STC-2; STCRP
• NCBI Protein Information: stanniocalcin-2
• UniProt Protein Name: Stanniocalcin-2
• Protein Family: Stanniocalcin
• UniProt Gene Name: STC2
• UniProt Synonym Gene Names: STC-2; STC-related protein; STCRP

NCBI Description

This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers. [provided by RefSeq, Jul 2008]

Uniprot Description

Has an anti-hypocalcemic action on calcium and phosphate homeostasis.