Goat TMP21/TMED10 Polyclonal Antibody | anti-TMED10 antibody

Goat anti-TMP21/TMED10 Antibody

Cat. Num. AAA420690

• Gene Names: TMED10; p23; TMP21; p24d1; S31I125; Tmp-21-I; S31III125; P24(DELTA)
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TMP21/TMED10; Polyclonal Antibody; Goat anti-TMP21/TMED10 Antibody; TMED10; transmembrane emp24-like trafficking protein 10 (yeast); P24(DELTA); S31I125; S31III125; TMP21; Tmp-21-I; 21 kDa transmembrane trafficking protein; transmembrane emp24 domain-containing protein 10; TMED10 antibody; transmembrane emp24-like trafficking protein 10 (yeast) antibody; P24(DELTA) antibody; S31I125 antibody; S31III125 antibody; TMP21 antibody; Tmp-21-I antibody; 21 kDa transmembrane trafficking protein antibody; transmembrane emp24 domain-containing protein 10 antibody; TMP21 / TMED10; anti-TMED10 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: YLRRFFKAKKLIE
• Sequence Length: 219

• Applicable Applications for anti-TMED10 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 1000.; Western Blot: Preliminary experiments gave an approx 75kDa band in Human and Rat Pancreas lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25. 0kDa according to NP_006818.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-YLRRFFKAKKLIE, from the C Terminus of the protein sequence according to NP_006818.3.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 98986464
• NCBI GeneID: 10972
• NCBI Accession #: NP_006818.3
• NCBI GenBank Nucleotide #: NM_006827.5
• Molecular Weight: 24,976 Da
• NCBI Official Full Name: transmembrane emp24 domain-containing protein 10
• NCBI Official Synonym Full Names: transmembrane p24 trafficking protein 10
• NCBI Official Symbol: TMED10
• NCBI Official Synonym Symbols: p23; TMP21; p24d1; S31I125; Tmp-21-I; S31III125; P24(DELTA)
• NCBI Protein Information: transmembrane emp24 domain-containing protein 10
• UniProt Protein Name: Transmembrane emp24 domain-containing protein 10
• Protein Family: Transmembrane protein
• UniProt Gene Name: TMED10
• UniProt Synonym Gene Names: TMP21; S31I125; p24delta1
• UniProt Entry Name: TMEDA_HUMAN

NCBI Description

This gene is a member of the EMP24/GP25L/p24 family and encodes a protein with a GOLD domain. This type I membrane protein is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. The protein is also a member of a heteromeric secretase complex and regulates the complex's gamma-secretase activity without affecting its epsilon-secretase activity. Mutations in this gene have been associated with early-onset familial Alzheimer's disease. This gene has a pseudogene on chromosome 8. [provided by RefSeq, Jul 2008]

Uniprot Description

TMED10: Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act togther with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus. Predominantly homodimeric and to lesser extent monomeric in endoplasmic reticulum. Homodimer and monomer in endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED7 and TMED9. Interacts with TMED2. Associates with the COPI vesicle coat (coatomer); TMED10:TMED2 heterotetramers are proposed to be involved in coatomer association. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED10. Interacts with ARF1 (GDP- bound); the interaction probably involves a TMED10 oligomer. Interacts with SEC23A; indicative for an association of TMED10 with the COPII vesicle coat. Interacts with CD59, SEC24B, SEC24C and SEC24D. Interacts with MPPE1/PGAP5. Interacts with F2LR1. Interacts with KDELR2; the interaction is disrupted by KDELR2 ligand. Found in a complex composed at least of SURF4, TMED2 and TMED10. Associates with the presenilin- dependent gamma-secretase complex. Ubiquitous. Belongs to the EMP24/GP25L family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: cis-Golgi network; COPI-coated vesicle; endoplasmic reticulum; endoplasmic reticulum membrane; ER-Golgi intermediate compartment; ER-Golgi intermediate compartment membrane; Golgi apparatus; Golgi membrane; integral to membrane; melanosome; plasma membrane; secretory granule membrane; trans-Golgi network transport vesicle; transport vesicle; zymogen granule membrane

Molecular Function: protein binding; protein complex binding; syntaxin binding

Biological Process: COPI coating of Golgi vesicle; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; Golgi organization and biogenesis; intracellular protein transport; kidney development; protein oligomerization; regulated secretory pathway; response to alkaloid; retrograde vesicle-mediated transport, Golgi to ER; vesicle targeting, to, from or within Golgi