Rabbit anti-Human TMEM5 Polyclonal Antibody | anti-TMEM5 antibody

TMEM5, NT (TMEM5, Transmembrane protein 5) (AP)

Cat. Num. AAA6356482

• Gene Names: RXYLT1; TMEM5; HP10481; MDDGA10
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TMEM5; Polyclonal Antibody; NT (TMEM5; Transmembrane protein 5) (AP); Transmembrane protein 5; anti-TMEM5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TMEM5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TMEM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-82 amino acids from the N-terminal region of human TMEM5.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TMEM5 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the TMEM5 antibody detected the TMEM5 protein (arrow).)

Product Categories/Family for anti-TMEM5 antibody

Antibodies; Transmembrane Proteins

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Yokoyama-Kobayashi, M., et al. Gene 228 (1-2), 161-167 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 7657178
• NCBI GeneID: 10329
• NCBI Accession #: NP_055069.1
• NCBI GenBank Nucleotide #: NM_014254.2
• UniProt Accession #: Q9Y2B1; Q6PKD6; A8K017
• Molecular Weight: 51,146 Da
• NCBI Official Full Name: ribitol-5-phosphate xylosyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: ribitol xylosyltransferase 1
• NCBI Official Symbol: RXYLT1
• NCBI Official Synonym Symbols: TMEM5; HP10481; MDDGA10
• NCBI Protein Information: ribitol-5-phosphate xylosyltransferase 1; UDP-D-xylose:ribitol-5-phosphate beta1,4-xylosyltransferase
• UniProt Protein Name: Ribitol-5-phosphate xylosyltransferase 1
• Protein Family: Transmembrane protein
• UniProt Gene Name: RXYLT1

NCBI Description

This gene encodes a type II transmembrane protein that is thought to have glycosyltransferase function. Mutations in this gene result in cobblestone lissencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]

Uniprot Description

UDP-xylosyltransferase involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:25279699, PubMed:27130732, PubMed:27733679, PubMed:27601598). Acts as a UDP-D-xylose:ribitol-5-phosphate beta1,4-xylosyltransferase, which catalyzes the transfer of UDP-D-xylose to ribitol 5-phosphate (Rbo5P) to form the Xylbeta1-4Rbo5P linkage on O-mannosyl glycan (PubMed:27130732, PubMed:27733679).