Rabbit TMEM41B Polyclonal Antibody | anti-TMEM41B antibody

TMEM41B (CT) Antibody

Cat. Num. AAA154819

• Reactivity: Human, Mouse, Rat; Predicted species reactivity based on immunogen sequence: Bovine (100%); Chicken (100%); Chimpanzee (100%).
• Applications: ELISA, Immunofluorescence, Western Blot
• Purity: TMEM41B Antibody is affinity chromatography purified via peptide column.
• Synonyms: TMEM41B; Polyclonal Antibody; TMEM41B (CT) Antibody; TMEM41B Antibody: Transmembrane protein 41B; Protein stasimon; KIAA0033; anti-TMEM41B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted species reactivity based on immunogen sequence: Bovine (100%); Chicken (100%); Chimpanzee (100%).
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TMEM41B Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; TMEM41B Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-TMEM41B antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: WB: 2ug/mL; IF: 20ug/mL.; ; Antibody validated: Western Blot in mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
• Immunogen: Anti-TMEM41B antibody was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human TMEM41B.; ; The immunogen is located within the last 50 amino acids of TMEM41B.
• Conjugation: Unconjugated
• Protein GI#: 74741383
• NCBI Organism: Homo sapiens
• Preparation and Storage: TMEM41B antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Figure 1 Western Blot Validation in Mouse Tissues Loading: 15 ug of lysates per lane.Antibodies: TMEM41B 9565, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

Western Blot (WB)

(Figure 2 Western Blot Validation in Rat TissuesLoading: 15 ug of lysates per lane. Antibodies: TMEM41B, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

Immunofluorescence (IF)

(Figure 3 Immunofluorescence Validation of TMEM41B in HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TMEM41B at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

Immunofluorescence (IF)

(Figure 4 Immunofluorescence Validation of TMEM41B in Human LungImmunofluorescent analysis of 4% paraformaldehyde-fixed human lung labeling TMEM41B at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

Immunofluorescence (IF)

(Figure 5 Immunofluorescence Validation of TMEM41B in Mouse Kidney Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney labeling TMEM41B with 9565 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

Immunofluorescence (IF)

(Figure 6 Immunofluorescence Validation of TMEM41B in Rat LungImmunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling TMEM41B at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

Related Product Information for anti-TMEM41B antibody

TMEM41B Antibody: TMEM41B is required for autophagosome formation and participates in early stages of autophagosome biogenesis at the ER membrane probably via mobilization of neutral lipids from lipid droplets. TMRM41B is a critical host factor required for infection by human coronaviruses SARS-CoV-2, HCoV-OC43, HCoV-NL63, and HCoV-229E, as well as all flaviviruses tested such as Zika virus and Yellow fever virus. It is required post-entry of the virus to facilitate the ER membrane remodeling necessary to form replication organelles.

Product Categories/Family for anti-TMEM41B antibody

Primary Antibody; Infectious Disease; COVID-19

References

Moretti et al. EMBO Rep. 2018; 19(9):e45889.
Shoemaker et al. PLoS Biol. 2019; 17(4):e2007044.
Schneider et al. Cell 2021; 184(1):120-132.
Hoffmann et al. Cell 2021; 184(1):133-148.e20.

NCBI and Uniprot Product Information

• NCBI GI #: 259013532
• NCBI GeneID: 440026
• NCBI Accession #: NP_001158502.1
• NCBI GenBank Nucleotide #: NM_001165030.2
• UniProt Accession #: Q5BJD5; Q15055; Q4G0P0; D3DQU9; E9PP29
• Molecular Weight: 32,513 Da
• NCBI Official Full Name: transmembrane protein 41B isoform 2
• NCBI Official Synonym Full Names: transmembrane protein 41B
• NCBI Official Symbol: TMEM41B
• NCBI Protein Information: transmembrane protein 41B
• UniProt Protein Name: Transmembrane protein 41B
• Protein Family: Transmembrane protein
• UniProt Gene Name: TMEM41B
• UniProt Synonym Gene Names: KIAA0033
• UniProt Entry Name: TM41B_HUMAN