Rabbit TMEM123 / Porimin Polyclonal Antibody | anti-TMEM123 antibody

Rabbit Polyclonal (IgG) to Human TMEM123 / Porimin

Cat. Num. AAA241868

• Gene Names: TMEM123; KCT3; PORMIN; PORIMIN
• Reactivity: Gibbon, Gorilla, Human, Monkey, Rabbit; Predicted Reactivity: Mouse, Rat, Hamster, Horse, Chicken (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: TMEM123 / Porimin; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human TMEM123 / Porimin; Anti-TMEM123 / Porimin Antibody (aa173-188) IHC-plus; TMEM123; KCT-3; KCT3; PORMIN; Transmembrane protein 123; Porimin; Serine/threonine-rich receptor; Human TMEM123; anti-TMEM123 antibody

• Host: Rabbit
• Reactivity: Gibbon, Gorilla, Human, Monkey, Rabbit; Predicted Reactivity: Mouse, Rat, Hamster, Horse, Chicken (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: A synthetic peptide corresponding to amino acids 173-188 SRRGIRYRTIDEHDAI of human Porimin; GenBank no. AAG2164.1. This sequence is 100% conserved in cow, and 93% conserved in rat, mouse, and chicken.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.05% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 208

• Applicable Applications for anti-TMEM123 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (1 - 3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 173-188 SRRGIRYRTIDEHDAIof human Porimin; GenBank no. AAG2164.1.
• Immunogen Type: Synthetic peptide
• Immunogen: TMEM123 / Porimin antibody was raised against this antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 173-188 SRRGIRYRTIDEHDAIof human Porimin; GenBank no. AAG2164.1. This sequence is 100% conserved in cow, and 93% conserved in rat, mouse, and chicken. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Rabbit (100%); Mouse, Rat, Hamster, Horse, Chicken (94%).
• Antigen Modification: aa173-188
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-TMEM123 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of Porimin using antibody at 2 ug/ml?dilution against 20 ug/lane of?HL60 cell lysate.)

NCBI and Uniprot Product Information

• NCBI GI #: 100913218
• NCBI GeneID: 114908
• NCBI Accession #: NP_443164.2
• NCBI GenBank Nucleotide #: NM_052932.2
• UniProt Accession #: Q8N131; Q8IWS2; Q96QV2
• Molecular Weight: 19,678 Da
• NCBI Official Full Name: porimin
• NCBI Official Synonym Full Names: transmembrane protein 123
• NCBI Official Symbol: TMEM123
• NCBI Official Synonym Symbols: KCT3; PORMIN; PORIMIN
• NCBI Protein Information: porimin; KCT-3; serine/threonine-rich receptor; pro oncosis receptor inducing membrane injury; pro-oncosis receptor inducing membrane injury; keratinocytes associated transmembrane protein 3; keratinocytes-associated transmembrane protein 3
• UniProt Protein Name: Porimin
• Protein Family: Porimin
• UniProt Gene Name: TMEM123
• UniProt Synonym Gene Names: KCT3; KCT-3
• UniProt Entry Name: PORIM_HUMAN

NCBI Description

This gene encodes a highly glycosylated transmembrane protein with a high content of threonine and serine residues in its extracellular domain, similar to a broadly defined category of proteins termed mucins. Exposure of some cell types to anti-PORIMIN (pro-oncosis receptor inducing membrane injury) antibody, crosslinks this protein on the cell surface and induces a type of cell death termed oncosis. Oncosis is distinct from apoptosis and is characterized by a loss of cell membrane integrity without DNA fragmentation. This gene product is proposed to function as a cell surface receptor that mediates cell death. [provided by RefSeq, Jul 2008]

Uniprot Description

TMEM123: Implicated in oncotic cell death, characterized by cell swelling, organelle swelling, vacuolization and increased membrane permeability. Belongs to the CD164 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q22.1

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: receptor activity