Western Blot (WB)
(Western blot analysis of extracts of U-87MG cells, using PUS1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human PUS1 Polyclonal Antibody | anti-PUS1 antibody
PUS1 Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of U-87MG cells, using PUS1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of U-87MG cells, using PUS1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]
Uniprot Description
PUS1: Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA. Defects in PUS1 are a cause of myopathy with lactic acidosis and sideroblastic anemia type 1 (MLASA1); also known as mitochondrial myopathy and sideroblastic anemia. MLASA is a rare autosomal recessive oxidative phosphorylation disorder specific to skeletal muscle and bone marrow. Belongs to the tRNA pseudouridine synthase TruA family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 5.4.99.12; RNA-binding; Isomerase; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; RNA processing
Chromosomal Location of Human Ortholog: 12q24.33
Cellular Component: mitochondrial matrix; mitochondrion; nuclear chromatin; nucleolus; nucleus; transcription factor complex
Molecular Function: ligand-dependent nuclear receptor transcription coactivator activity; pseudouridine synthase activity; pseudouridylate synthase activity
Biological Process: gene expression; positive regulation of transcription from RNA polymerase II promoter; tRNA modification; tRNA processing; tRNA pseudouridine synthesis
Disease: Myopathy, Lactic Acidosis, And Sideroblastic Anemia 1
Research Articles on PUS1
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Product Notes
The PUS1 pus1 (Catalog #AAA9130469) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PUS1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PUS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:200 - 1:2000, IF: 1:10 - 1:100. Researchers should empirically determine the suitability of the PUS1 pus1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PUS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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