Rabbit anti-Human TM173 Polyclonal Antibody | anti-TM173 antibody

TM173, CT (TMEM173, ERIS, MITA, STING, Transmembrane protein 173, Endoplasmic reticulum interferon stimulator, Mediator of IRF3 activation, Stimulator of interferon genes protein) (Azide free) (HRP)

Cat. Num. AAA6356013

• Gene Names: TMEM173; ERIS; MITA; MPYS; NET23; STING
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TM173; Polyclonal Antibody; CT (TMEM173; ERIS; MITA; STING; Transmembrane protein 173; Endoplasmic reticulum interferon stimulator; Mediator of IRF3 activation; Stimulator of interferon genes protein) (Azide free) (HRP); TMEM173; Stimulator of interferon genes protein; anti-TM173 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TM173 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TM173 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318~348 amino acids from the C-terminal region of human TM173.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TM173 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). TM173 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(TM173 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TM173 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TM173 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TM173 antibody

TM173 acts as a facilitator of innate immune signaling. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon (IFN-alpha and IFN-beta) and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.

Product Categories/Family for anti-TM173 antibody

Antibodies; Transmembrane Proteins

References

Sun, W., et al. Proc. Natl. Acad. Sci. U.S.A. 106(21):8653-8658(2009). Zhong, B., et al. Immunity 30(3):397-407(2009). Graubert, T.A., et al. PLoS ONE 4 (2), E4583 (2009). Zhong, B., et al. Immunity 29(4):538-550(2008). Ishikawa, H., et al. Nature 455(7213):674-678(2008). Jin, L., et al. Mol. Cell. Biol. 28(16):5014-5026(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 38093659
• NCBI GeneID: 340061
• NCBI Accession #: NP_938023.1
• NCBI GenBank Nucleotide #: NM_198282.2
• UniProt Accession #: Q86WV6; A8K3P6; B6EB35; D6RBX0; D6RE01; D6RID9
• Molecular Weight: 379
• NCBI Official Full Name: stimulator of interferon genes protein
• NCBI Official Synonym Full Names: transmembrane protein 173
• NCBI Official Symbol: TMEM173
• NCBI Official Synonym Symbols: ERIS; MITA; MPYS; NET23; STING
• NCBI Protein Information: stimulator of interferon genes protein; hMITA; hSTING; mediator of IRF3 activation; endoplasmic reticulum IFN stimulator; mitochondrial mediator of IRF3 activation; endoplasmic reticulum interferon stimulator; N-terminal methionine-proline-tyrosine-serine
• UniProt Protein Name: Stimulator of interferon genes protein
• UniProt Gene Name: TMEM173
• UniProt Synonym Gene Names: ERIS; MITA; STING; hSTING; ERIS; hMITA
• UniProt Entry Name: STING_HUMAN