Rabbit anti-Mouse, Human TLR6 Polyclonal Antibody | anti-TLR6 antibody

TLR6, CT (Toll-like Receptor 6, CD286) (Biotin)

Cat. Num. AAA6502984

• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: TLR6; Polyclonal Antibody; CT (Toll-like Receptor 6; CD286) (Biotin); anti-TLR6 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse TLR6. Species Crossreactivity: human
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TLR6 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 722-753 in the C-terminal region of human TLR6, UniProt Accession #Q9EPW9
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) using T8050-55F. TLR6 (arrow) was detected using T8050-55F.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue using T8050-55F, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of T8050-55F for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human lung carcinoma tissue using T8050-55F, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of T8050-55F for immunohistochemistry.)

Product Categories/Family for anti-TLR6 antibody

Antibodies; Toll Like Receptors (TLR)

References

1. Ozinsky, A., et al., Proc. Natl. Acad. Sci. USA 97(25):13766-13771 (2000). 2. Takeuchi, O., et al., Gene 231(1-2), 59-65 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 157057101
• NCBI GeneID: 21899
• NCBI Accession #: NP_035734.3
• NCBI GenBank Nucleotide #: NM_011604.3
• UniProt Accession #: Q9EPW9; Q9WTQ4
• Molecular Weight: 91,116 Da
• NCBI Official Full Name: toll-like receptor 6
• NCBI Official Synonym Full Names: toll-like receptor 6
• NCBI Official Symbol: Tlr6
• NCBI Protein Information: toll-like receptor 6
• UniProt Protein Name: Toll-like receptor 6
• UniProt Gene Name: Tlr6

Uniprot Description

Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides (PubMed:19931471). In response to diacylated lipopeptides, forms the activation cluster TLR2:TLR6:CD14:CD36, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. In complex with TLR4, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion (PubMed:20037584, PubMed:23812099).