Rabbit TLR5 Polyclonal Antibody | anti-TLR5 antibody

Rabbit Polyclonal (IgG) to Human TLR5

Cat. Num. AAA242775

• Gene Names: TLR5; SLE1; TIL3; SLEB1; MELIOS
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein G Purified
• Synonyms: TLR5; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human TLR5; Anti-TLR5 Antibody (aa300-350) IHC-plus; TIL3; Toll-like receptor 5; SLEB1; Human TLR5; anti-TLR5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 found between amino acids 300-350. It will cross-react with mouse and rat TLR5.
• Purity/Purification: Protein G Purified
• Form/Format: PBS containing 0.05% BSA and 0.05% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 858

• Applicable Applications for anti-TLR5 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo, IHC-P (10 ug/ml), WB (1 - 3 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human TLR5.
• Immunogen Type: Synthetic peptide
• Immunogen: TLR5 antibody was raised against synthetic peptide from human TLR5.
• Antigen Modification: aa300-350
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-TLR5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Flow Cytometry (FC/FACS)

(Intracellular flow analysis of TLR5 in Balb/c mouse splenocytes using TLR5 antibody at 2 ug/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control; red represents TLR5 antibody. Goat anti-rabbit PE was used as the secondary antibody.)

Western Blot (WB)

(Western blot of TLR5 in (A) Ramos, (B) Raw, (C) mouse spleen, (D) mouse lung and (E) rat lung whole cell lysate using antibody at 2 ug/ml.)

Product Categories/Family for anti-TLR5 antibody

Family: Toll-like Receptor

NCBI and Uniprot Product Information

• NCBI GI #: 16751843
• NCBI GeneID: 7100
• NCBI Accession #: NP_003259.2
• NCBI GenBank Nucleotide #: NM_003268.5
• UniProt Accession #: O60602; O15456; Q32MI2; Q32MI3; B1AZ05; B3Y633; B9VJ63; D1CS80; D3DTB8
• Molecular Weight: 97,834 Da
• NCBI Official Full Name: toll-like receptor 5
• NCBI Official Synonym Full Names: toll-like receptor 5
• NCBI Official Symbol: TLR5
• NCBI Official Synonym Symbols: SLE1; TIL3; SLEB1; MELIOS
• NCBI Protein Information: toll-like receptor 5; toll/interleukin-1 receptor-like protein 3; systemic lupus erythematosus susceptibility 1
• UniProt Protein Name: Toll-like receptor 5
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR5
• UniProt Synonym Gene Names: TIL3
• UniProt Entry Name: TLR5_HUMAN

NCBI Description

This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009]

Uniprot Description

TLR5: Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Genetic variation in TLR5 is associated with resistance to systemic lupus erythematosus type 1 (SLEB1). Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q41-q42

Cellular Component: integral to membrane; plasma membrane

Molecular Function: transmembrane receptor activity; interleukin-1 receptor binding

Biological Process: positive regulation of interleukin-8 production; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of cytokine secretion; positive regulation of nitric oxide biosynthetic process; defense response to bacterium; male gonad development; toll-like receptor signaling pathway; innate immune response; inflammatory response; positive regulation of toll-like receptor signaling pathway; toll-like receptor 10 signaling pathway

Disease: Legionnaire Disease, Susceptibility To; Systemic Lupus Erythematosus, Susceptibility To, 1; Melioidosis, Susceptibility To