Rabbit anti-Mouse, Rat TLR13 Polyclonal Antibody | anti-TLR13 antibody
TLR13 Antibody FITC-Conjugated
Gene Names
Tlr13; Gm713; AI666735
Reactivity
Mouse, Rat
Applications
ELISA, Immunoprecipitation, Western Blot
Synonyms
TLR13; Polyclonal Antibody; TLR13 Antibody FITC-Conjugated; Toll like receptor 13 antibody; anti-TLR13 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Form/Format
FITC-Conjugated Immunoglobulins
Concentration
0.75 mg/mL IgG in antibody stabilization buffer (varies by lot)
Sequence Length
991
Applicable Applications for anti-TLR13 antibody
ELISA, IP , WB
Application Notes
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500
Immunogen
Synthetic peptide taken within amino acid region 400-480 of mouse TLR13 protein.
Molecular Function
protein homodimerization activity, rRNA binding
Subcellular Location
endosome membrane, Single-pass type I membrane protein
Preparation and Storage
Store at -20°C for long term storage.
Related Product Information for anti-TLR13 antibody
Overview:
The Toll-like receptor (TLR) multigene family encodes important recognition receptors of the innate immune system that have been conserved in both the invertebrate and vertebrate lineages. The mammalian host defense system is essentially regulated by these conserved Toll-like receptors. At least 13 TLRs have been identified and cloned in mammalian cells which recognize molecular products/signals from all the major classes of pathogens and activation of innate immunity. TLRs were identified as genes coding for both an N-terminal leucine-rich repeat (LRR) domain and a C-terminal Toll-IL-resistance (TIR) domain. The TLRs form families of ligand-binding proteins that engage a variety of microbial products, which on binding, activate signaling cascades leading to the synthesis of pro inflammatory molecules. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. The Toll signaling to NF-Kb starts form conserved Toll-;-resistance (TiR) domain, which mediated the coupling of TIR adaptor molecules (MyD88, Mal, TICAM and TRAM) and caused production of inflammatory cytokines such as IL-1, IL-6, IL-8 , TNFa, and IL-12, chemokines and co-stimulatory molecules such as CD40, CD80 and CD86. TLR induced apoptosis pathway is a repertoire of defense mechanism utilized by innate defense mechanism. The constitutive expression of many human TLRs (TLR1, TLR2, and TLR3) have been shown on the surface of myeloid lineage cells by RT-PCR and use of specific monoclonal antibodies. The expression of TLR 3, TLR 7, TLR 8 and TLR 9 are mainly found on endosomal lysosomal compartments. There is significant evidence of TLR involvement is many systemic disorders following bacterial infection including sepsis, peridontitis, cardiac ischemia, cerebral palsy and others. Understanding the TLRs involvement in these conditions will allow therapeutic interventions at the receptor level for treatment of these disorders. The TLR are highly conserved protein and share structural and functional domains across species. These receptors recognize pathogen associated molecular patters (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines needed for production of immediate immunity. The cell-surface TLRs, including TLR1. TLR2, TLR4, and TLR6, recognize microbial membrane lipids, whereas TLR3, TLR7, TLR8, and TLR9 recognize pathogen-derived nucleotides in intracellular compartments. TLR7 and TLR9 respond to host-derived nucleotides as well, and they have been implicated in a variety of autoimmune diseases. Toll-like receptor 9 (TLR9) is a receptor for methylated CpG dinucleotides found in bacterial and viral DNA. TLR11-TLR13 and TLR21-TLR23 subfamilies are represented by pseudogenes in humans. TLR11-TLR13 are important targets in central nervous system infection.
TLR13 is a member of the Toll-like receptor family of trans membrane proteins comprised of multiple copies of leucine rich repeats in the extracellular domain and a TIR domain. This 991 amino acid containing protein is closely related to TLR3 and participates in the innate immune response to microbial agents. TLR13 binds to and acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and inflammatory response. TLR13 functions as a component of innate and adaptive immunity that recognizes and binds 23S rRNA from bacteria. It specifically binds the 5'-CGGAAAGACC-3' sequence on bacterial 23S rRNA, a sequence also bound by MLS group antibiotics (including erythromycin). TLR13 is not conserved in humans. Humans may instead possess a related rRNA-sensing pattern recognition receptor that has evolved to recognize species that can hide from TLR13 owing to rRNA modifications. TLR13-selective antibodies were generated against a peptide taken from mouse TLR13 protein ranging from amino acids 400-480.
The TLR13-selective antibodies are affinity purified on an immobilized antigen based affinity matrix. The isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The TLR13- selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. Western blot positive control samples in "ready-to-use" SDS-PAGE sample buffer and antigenic blocking peptide for TLR13 are available.
The Toll-like receptor (TLR) multigene family encodes important recognition receptors of the innate immune system that have been conserved in both the invertebrate and vertebrate lineages. The mammalian host defense system is essentially regulated by these conserved Toll-like receptors. At least 13 TLRs have been identified and cloned in mammalian cells which recognize molecular products/signals from all the major classes of pathogens and activation of innate immunity. TLRs were identified as genes coding for both an N-terminal leucine-rich repeat (LRR) domain and a C-terminal Toll-IL-resistance (TIR) domain. The TLRs form families of ligand-binding proteins that engage a variety of microbial products, which on binding, activate signaling cascades leading to the synthesis of pro inflammatory molecules. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. The Toll signaling to NF-Kb starts form conserved Toll-;-resistance (TiR) domain, which mediated the coupling of TIR adaptor molecules (MyD88, Mal, TICAM and TRAM) and caused production of inflammatory cytokines such as IL-1, IL-6, IL-8 , TNFa, and IL-12, chemokines and co-stimulatory molecules such as CD40, CD80 and CD86. TLR induced apoptosis pathway is a repertoire of defense mechanism utilized by innate defense mechanism. The constitutive expression of many human TLRs (TLR1, TLR2, and TLR3) have been shown on the surface of myeloid lineage cells by RT-PCR and use of specific monoclonal antibodies. The expression of TLR 3, TLR 7, TLR 8 and TLR 9 are mainly found on endosomal lysosomal compartments. There is significant evidence of TLR involvement is many systemic disorders following bacterial infection including sepsis, peridontitis, cardiac ischemia, cerebral palsy and others. Understanding the TLRs involvement in these conditions will allow therapeutic interventions at the receptor level for treatment of these disorders. The TLR are highly conserved protein and share structural and functional domains across species. These receptors recognize pathogen associated molecular patters (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines needed for production of immediate immunity. The cell-surface TLRs, including TLR1. TLR2, TLR4, and TLR6, recognize microbial membrane lipids, whereas TLR3, TLR7, TLR8, and TLR9 recognize pathogen-derived nucleotides in intracellular compartments. TLR7 and TLR9 respond to host-derived nucleotides as well, and they have been implicated in a variety of autoimmune diseases. Toll-like receptor 9 (TLR9) is a receptor for methylated CpG dinucleotides found in bacterial and viral DNA. TLR11-TLR13 and TLR21-TLR23 subfamilies are represented by pseudogenes in humans. TLR11-TLR13 are important targets in central nervous system infection.
TLR13 is a member of the Toll-like receptor family of trans membrane proteins comprised of multiple copies of leucine rich repeats in the extracellular domain and a TIR domain. This 991 amino acid containing protein is closely related to TLR3 and participates in the innate immune response to microbial agents. TLR13 binds to and acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and inflammatory response. TLR13 functions as a component of innate and adaptive immunity that recognizes and binds 23S rRNA from bacteria. It specifically binds the 5'-CGGAAAGACC-3' sequence on bacterial 23S rRNA, a sequence also bound by MLS group antibiotics (including erythromycin). TLR13 is not conserved in humans. Humans may instead possess a related rRNA-sensing pattern recognition receptor that has evolved to recognize species that can hide from TLR13 owing to rRNA modifications. TLR13-selective antibodies were generated against a peptide taken from mouse TLR13 protein ranging from amino acids 400-480.
The TLR13-selective antibodies are affinity purified on an immobilized antigen based affinity matrix. The isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The TLR13- selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. Western blot positive control samples in "ready-to-use" SDS-PAGE sample buffer and antigenic blocking peptide for TLR13 are available.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Toll-like receptor 13
NCBI Official Synonym Full Names
toll-like receptor 13
NCBI Official Symbol
Tlr13
NCBI Official Synonym Symbols
Gm713; AI666735
NCBI Protein Information
toll-like receptor 13
UniProt Protein Name
Toll-like receptor 13
Protein Family
UniProt Gene Name
Tlr13
UniProt Entry Name
TLR13_MOUSE
Uniprot Description
TLR13: Component of innate and adaptive immunity that recognizes and binds 23S rRNA from bacteria. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Specifically binds the 5'-CGGAAAGACC-3' sequence on bacterial 23S rRNA, a sequence also bound by MLS group antibiotics (including erythromycin). May also recognize vesicular stomatitis virus; however, these data require additional evidences. Belongs to the Toll-like receptor family.
Protein type: Receptor, misc.; Membrane protein, integral
Cellular Component: cytoplasm; endosome; integral to membrane; membrane
Molecular Function: double-stranded RNA binding; protein binding; protein homodimerization activity; RNA binding; rRNA binding
Biological Process: immune system process; inflammatory response; innate immune response; MyD88-dependent toll-like receptor signaling pathway; regulation of MAPKKK cascade; response to virus; signal transduction; toll-like receptor 13 signaling pathway
Research Articles on TLR13
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Product Notes
The TLR13 tlr13 (Catalog #AAA5400088) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TLR13 Antibody FITC-Conjugated reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TLR13 can be used in a range of immunoassay formats including, but not limited to, ELISA, IP, WB. Dot Blot: 1:10,000 ELISA: 1:10,000 Immunoprecipitation: 1:200 Western Blot: 1:500. Researchers should empirically determine the suitability of the TLR13 tlr13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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