Rabbit anti-Human, Mouse TLR1 Polyclonal Antibody | anti-TLR1 antibody

Anti-TLR1 Antibody

Cat. Num. AAA419494

• Gene Names: TLR1; TIL; CD281; rsc786; TIL. LPRS5
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: TLR1; Polyclonal Antibody; Anti-TLR1 Antibody; anti-TLR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of various cell lines, using TLR1 antibody. Immunohistochemistry of paraffin-embedded human stomach cancer using TLR1 antibody at dilution of 1:200 (200x lens). Immunofluorescence analysis of HeLa cell using TLR1 antibody. Blue: DAPI for nuclear staining.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 786

• Applicable Applications for anti-TLR1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200; IF: 1:50- 1:200
• Immunogen: Recombinant protein of human TLR1
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunofluorescence (IF)

Related Product Information for anti-TLR1 antibody

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-B and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IB that normally maintains NF-B inactivity by sequestering it in the cytoplasm. Toll-like receptor expression is highest in peripheral blood leukocytes, monocytes, macrophages, though TLR1 expression may be less restricted than other family members (4,5). TLR1 associates with TLR2 to cooperatively mediate immune responses to bacterial lipoproteins and lead to NF-B activation (6, 7). TLR1 shows highest homology to TLR6, which shares 69% sequence identity (8).

NCBI and Uniprot Product Information

• NCBI GI #: 49065550
• NCBI GeneID: 7096
• Molecular Weight: 90,291 Da
• NCBI Official Full Name: TLR1
• NCBI Official Synonym Full Names: toll like receptor 1
• NCBI Official Symbol: TLR1
• NCBI Official Synonym Symbols: TIL; CD281; rsc786; TIL. LPRS5
• NCBI Protein Information: toll-like receptor 1
• UniProt Protein Name: Toll-like receptor 1
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR1
• UniProt Synonym Gene Names: KIAA0012; TIL

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR1: Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Binds MYD88 (via TIR domain). Interacts with CNPY3. Interacts (via extracellular domain) with TLR2. Ligand binding induces the formation of a heterodimer with TLR2. Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: Golgi apparatus; integral to plasma membrane; lipid raft; plasma membrane

Molecular Function: protein binding; protein heterodimerization activity; receptor activity; transmembrane receptor activity

Biological Process: defense response to bacterium; detection of triacylated bacterial lipoprotein; immune response; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-6 biosynthetic process; positive regulation of tumor necrosis factor biosynthetic process; signal transduction; toll-like receptor signaling pathway

Disease: Leprosy, Susceptibility To, 5