FHL3 recombinant protein

FHL3, 1-280aa, Human, His tag, E Coli

Cat. Num. AAA204811

• Gene Names: FHL3; SLIM2
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: FHL3; 1-280aa; Human; His tag; E Coli; Four and a half LIM domains 3; SLIM2; NP_004459; SLIM-2; Skeletal muscle LIM-protein 2; FHL3 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSESFDC AKCNESLYGR KYIQTDSGPY CVPCYDNTFA NTCAECQQLI GHDSRELFYE DRHFHEGCFR CCRCQRSLAD EPFTCQDSEL LCNDCYCSAF SSQCSACGET VMPGSRKLEY GGQTWHEHCF LCSGCEQPLG SRSFVPDKGA HYCVPCYENK FAPRCARCSK TLTQGGVTYR DQPWHRECLV CTGCQTPLAG QQFTSRDEDP YCVACFGELF APKCSSCKRP IVGLGGGKYV SFEDRHWHHN CFSCARCSTS LVGQGFVPDG DQVLCQGCSQ AGP
• Sequence Length: 280

• Applicable Applications for FHL3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for FHL3 recombinant protein

FHL3 is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human FHL3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for FHL3 recombinant protein

Epigenomics

References

Morgan M.J., et al. (1999) Biochem. Biophys. Res. Commun. 255:245-250; Morgan M.J., et al. (1996) Biochem. Biophys. Res. Commun. 225:632-638;

NCBI and Uniprot Product Information

• NCBI GI #: 54112385
• NCBI GeneID: 2275
• NCBI Accession #: NP_004459
• NCBI GenBank Nucleotide #: NM_004468.4
• UniProt Accession #: Q13643; Q6I9T0; Q9BVA2; D3DPT6
• Molecular Weight: 33.6 kDa (303aa) confirmed by MALDI-TOF
• NCBI Official Full Name: four and a half LIM domains protein 3 isoform 1
• NCBI Official Synonym Full Names: four and a half LIM domains 3
• NCBI Official Symbol: FHL3
• NCBI Official Synonym Symbols: SLIM2
• NCBI Protein Information: four and a half LIM domains protein 3
• UniProt Protein Name: Four and a half LIM domains protein 3
• Protein Family: Four and a half LIM domains protein
• UniProt Gene Name: FHL3
• UniProt Synonym Gene Names: SLIM2; FHL-3; SLIM-2

NCBI Description

The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]